The file /Demo_Images/ZAMBIA_FIBERS.tiff is an AEM image of asbestos fibers on a Nuclepore filter.

• Load the image {MLx - image files - TIFF - read}.
• Display the image by using the show button, or clicking on the zoom box.
• Try histogram equalization using the scale - equalize button in the Top Image collumn of the MLX buttons window. This visualizes the filter material rather than the fibers.

Instructions for histogram equalization in NIH Image:

• Open the image with the {file - open} menu.
• Enhance with the {Process - Equalize} menu.
• Compare results with the {Process - Enhance Contrast} menu which is a linear contrast stretch.

Instructions for histogram equalization in Adobe Photoshop

• Open the image with the file - open menu.
• Enhance with the Image - Map - Equalize menu.