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Publication Citation: Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes

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Author(s): Dustin Levy; Kimberly A. Briggman;
Title: Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes
Published: May 25, 2007
Abstract: The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder transitions of the lipid acyl chains was used to determine the main gel to liquid crystal phase transition temperature, Tm, for HBMs of binary cholesterol/phospholipid mixtures on octadecanethiolate self-assembled monolayers. The phase transition temperature and the breadth of the transition were shown to increase with cholesterol content, and the phase boundaries observed in the cholesterol/phospholipid HBMs were comparable to the published phase diagrams of binary cholesterol/phospholipid vesicles. A thermodynamic assessment of the cooperative units of theHBMphase transitions revealed the presence of<10nmdiameter domains that were independent of the cholesterol composition.
Citation: Biophysical Journal
Volume: 23
Pages: pp. 7155 - 7161
Keywords: cholesterol-lipid phase diagrams;hybrid bilayer membranes;SFG;supported bilayer membrane mimics;vibrational sum frequency
Research Areas: Biological Physics, Nonlinear Optical Spectroscopy, Bioscience & Health, Optical Technology
PDF version: PDF Document Click here to retrieve PDF version of paper (130KB)