| Author(s): |
Gregg M. Gallatin; Andrew J. Berglund; |
| Title: |
Optimal Laser Scan Path for Localizing a Fluorescent Particle in Two or Three Dimensions |
| Published: |
July 16, 2012 |
| Abstract: |
Localizing a fluorescent particle by scanning a focused laser in its vicinity and analyzing the detected photon stream provides real-time information for a modern class of feedback control systems for particle tracking and trapping. We present a simple mathematical test to determine whether a particular laser scan path encodes maximal information about a fluorescent particle's position in the detected photon stream. We give the optimal path for a Gaussian laser scanned in two or three dimensions, and present the Cram\'{e}r-Rao bound on the two and three-dimensional localization accuracy that can be achieved by analyzing the detected photon stream. |
| Citation: |
Optics Express |
| Volume: |
20 |
| Issue: |
15 |
| Pages: |
pp. 16381 - 16393 |
| Keywords: |
single particle tracking |
| Research Areas: |
Optics, Optical microscopy |
| PDF version: |
 Click here to retrieve PDF version of paper (842KB) |
