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Cell Systems Science Group

Welcome

We are entering an era in which Biology is rapidly becoming an information science. Advances in measurement technologies have provided insight into how complex biological processes can be, and have allowed us to begin to explore the vast parameter space that determines biological outcomes. This New Biology has its foundation in physical measurements, and requires quantitative analytical rigor.

The Cell Systems Science Group contributes to the new post-genomic era with research in measurements and models that support the understanding of complex biological phenomena at the cellular and subcellular level through bioimaging, measurement of protein interactions, microfluidics, and bioinformatics. We do this by improving and developing methods for measurements, data analysis, and data handling. These efforts assist basic biomedical research, biotechnologies such as biofuels and drug development, and other applications that depend on understanding and predicting complex cellular activities.

Cell Systems Science Group Cell Culture

Programs/Projects

Fluorescence Microscope Benchmarking—We have made available a method to characterize a fluorescence microscope's performance by benchmarking the detection threshold, saturation and linear dynamic range to a physical artifact (i.e. a …

Collagen Films Protocol—Background: Collagen type 1 is the most prevalent extracellular protein in connective tissue, and is part of the family of collagens that are fibril forming. Neutralizing monomeric collagen …

Assessing NP EHS—Due to their small size, nanoparticles can enter living organisms in previously unforeseen ways and their immense surface area results in higher reactivity compared to corresponding bulk materials. …

Development of a Collagen Fibril Reference Material for Reproducible Cell Response—An important determinant of cellular response to drugs and toxins is the chemical and physical nature of the extracellular matrix (ECM) to which cells are adhered to within tissues. The cell’s ECM …

Evaluating Measurement Quality of Microfluidic Cell-based Assays—Microfluidic technologies show promise as miniaturized, portable platforms for cell assays, but must be tested to establish confidence of within-device consistency, assurance of day-to-day …

Development of Biomarker-based Systems for Cancer Detection and Therapy— Biomarkers play an increasingly important role in disease detection and treatment. As one of the Biomarker Reference Laboratories (BRLs) in the Early Detection Research Network (EDRN) - an …

Biocompatibility of Photoluminescent Silicon Nanoparticles—Photoluminescent silicon nanoparticles have a bright and stable fluorescence and are promising candidates for use in bio-imaging, cell staining and drug delivery. In collaboration with the FDA …

Ontologies and Interoperability in Evolutionary Comparative Analysis—Many computer-based inferences in contemporary biology are comparative and would benefit from the rigorous and flexible approach of evolutionary comparative analysis (ECA), in which similarities …

Characterization of Dolphin Lung Endothelial Cells Through Quantitative Imaging—A collaboration between the Medical University of South Carolina (MUSC), Hollings Marine Laboratory, and NIST is examining the relationship between cell phenotype and gene expression in lung …

Multiparametric Measurement and Analysis of Cell Behavior on Extracellular Matrices—Cell responses to their culture environment always involve more than one component. An understanding of the relation between the physical and chemical features of the extracellular matrix (ECM) and …

Surface Plasmon Resonance Imaging of Cells and Extracellular Matrix Proteins—A critical challenge in cell biology is imaging and quantifying the interactions of cells with their extracellular matrix (ECM) environment, and the active remodeling by cells of their ECM. …

Structural Databases—We are witnessing the emergence of a web based "data rich" era on chemical and biological compounds. In order to make significant advances in this "data rich" era, it is essential that there be …

Standards for Immuno-Histochemical Imaging: A Biomarker Protein Expression Chip—Immunohistochemistry (IHC) is used to visualize antigens in tissue sections by specific antigen-antibody interactions, an advantage over traditionally used staining techniques that can identify …

Protection Against Ionizing Radiation in Extreme Radiation-Resistant Microorganisms— We measured the levels of modified DNA bases following ?-irradiation of the halophilic archaeon, Halobacterium sp. str. NRC-1. In this first study to quantify DNA base modification in a …

Improved Benchtop Connectivity to Complex Microfluidic Devices—Despite their promise to enable high-throughput science and discovery, microfluidic devices have not yet been broadly adopted. An important missing element in current microfluidic technology is a …

Fundamental Interaction Mechanisms of Engineered Nanomaterials with DNA— We utilized isotope-dilution liquid chromatography/mass spectrometry (LC/MS) to determine the levels of the oxidatively damaged DNA bases, 8-hydroxydeoxyguanosine (8-OH-dG) and …

Development of SRMs for Serum Proteomics—Serum proteomics, the detection and measurement of as many proteins in a serum sample as possible, could be an important element of personalized medicine enabling diagnosis of early stages of …

Cell-based Immunofluorescence Assays—Microscopy images of cells are often used in cell biology to qualitatively identify features of interest and to discern differences between treatments. Images are also sometimes used to quantify …

Cell-based Assay for Mechanistically-based Prediction of Cytoxicity—The most commonly used cell-based assays for measuring cytotoxicity often show poor reproducibility, require 24 h to complete, and rely on a nonspecific response, namely cell death. Better assay …

Automated Live Cell Imaging for Quantification of Catalytic Rates in Individual Cells—To accurately interpret the data from fluorescent proteins as reporters of gene activation within living cells, it is important to understand the kinetics of the degradation of the reporter …