This project leverages the skills and resources of the Hollings Marine Laboratory (HML) partnership that includes researchers with expertise and experience on the biology of a wide variety of marine organisms from microbes to marine mammals coupled with unique high-field nuclear magnetic resonance (NMR). The application of metabolomics techniques which have enjoyed widespread application in human health research to marine environmental research presents a novel opportunity to influence and contribute to new understanding of non-target organism systems biology, to the development of new tools for environmental modeling and risk assessment, to the enhancement of multivariate measurement protocols and standards and to the development of ecosystem-scale bio-measurement strategies.
A small number of labs throughout the world are in a position to conduct these types of studies, and because of the nature of NMR-based metabolomics studies, important contributions to systems biology through synergy with proteomics and genomics studies are possible.
Additional Technical Details:
Start Date:October 1, 2007
Lead Organizational Unit:mml
Related Programs and Projects:
Fundamental Chemical Metrology (Inorganic/Organic/Gas Metrology)
N. Morrison, D. Bearden, J. G. Bundy, T. Collette, F. Currie, M. P. Davey, N. S. Haigh, D. Hancock, O. A. H. Jones, S. Rochfort, S. A. Sansone, D. Stys, Q. Teng, D. Field, M. R. Viant: Standard Reporting Requirements for Biological Samples in Metabolomics Experiments: Environmental Context, Metabolomics3: 203-210 (2007).
J. E. Oatis, Jr., P. Brunsfeld, J. W. Rushing, P. D. Moeller, D. W. Bearden, T. N. Gallien, and G. Cooper, IV: Simplified Isolation and Purification of Cyclopamine from Veratrum californicum, Chemistry Central Journal. 2(12): On-line (2008).
M. R. Viant, D. W. Bearden, J. G. Bundy, I. W. Burton, T. W. Collette, D. R. Ekman, V. Ezernieks, T. K. Karakach, C. Yu Lin, S. Rochfort, J. S. de Ropp, Q. Teng, R. S. Tjeerdema, J. A. Walter, and H. Wu: International NMR-Based Environmental Metabolomics Intercomparison Exercise, Environmental Science & Technology, 43(1), 219-225 (2009).
B. A. Neely, D. W. Bearden, A. J. Sutter, E. C. Pollock, N. J. Kabengi, P. M. Bertsch, and P. J. Morris: Cytotoxicity of Engineered ZnO Nanoparticles to Burkholderia vietnamiensis PR1301: Comparison to Zn2+ and the Effects of Counter-ion Utilization, Submitted to Environmental Science & Technology (2009).
T. B. Schock, K. G. Burnett, L. E. Burnett, L. Thibodeaux, D. A. Stancyk, D. W. Bearden: Metabolomic Analysis of Atlantic Blue Crab, Callinectes sapidus, Hemolymph Following Oxidative Stress, in preparation.
A. F. B. Boroujerdi, A. Meyers, E. C. Pollock, S. L. Huynh, M. Vizcaino, P. J. Morris, and D. W. Bearden: NMR-Based Microbial Metabolomics and the temperature-dependent coral pathogen Vibrio coralliilyticus, in preparation.