Additional Technical Details:
The Micronutrients Measurement QA Program at NIST has administered over 90 interlaboratory comparison exercises for the measurement of selected fat- and water-soluble vitamins in human serum and plasma. The comparability of laboratory measurements for target analytes has improved substantially over time. This has been achieved through the development and promulgation of robust measurement technologies, identification and production of suitable reference materials, isolation and identification of measurement system biases, and support and encouragement of long-term within-laboratory measurement quality control efforts. The average estimated coefficient of variation for retinol and α-tocopherol has been approximately 5% for the past seven years and about 10% for β-carotene for that same period of time.
For program information, including how to join, click here.
Start Date:October 1, 1984
Lead Organizational Unit:mml
ARUP Laboratories, Salt Lake City, UT, USA
Related Programs and Projects:
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Brown Thomas, J. and Sharpless, K. E., eds., Methods for Analysis of Cancer Chemopreventive Agents in Human Serum, NIST Special Publication 874, U. S. Gov't Printing Office, Washington, DC, 128 pages, (1995).
Sharpless, K. E.; Duewer, D. L. Population Distributions and Intralaboratory Variance for Fat-Soluble Vitamin-Related Compounds in Human Serum, Anal. Chem., 67: 4416-4422 (1995).
Brown Thomas, J.; Kline, M. C.; Schiller, S. B.; Ellerbe, P. M.; Sniegoski, L. T.; Duewer, D. L.; Sharpless, K. E. Certification of Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum: Standard Reference Material 968b, Fresenius' J. Anal. Chem., 356:1-9 (1996).
Margolis, S. A.; Duewer, D. L. The Measurement of Ascorbic Acid in Human Plasma and Serum: Intralaboratory Repeatability, Interlaboratory Repeatability, Interlaboratory Reproducibility, and Stability in Lyophilized Plasma and Metaphosphoric Acid Preserved Plasma and Serum, Clin. Chem., 42: 1257-1262 (1996).
Duewer, D.L., Brown Thomas, J. Kline, M.C., MacCrehan, W. A., Schaffer, R., Sharpless, K.E., May, W.E., “NIST/NCI Micronutrients Measurement Quality Assurance Program: Measurement Reproducibility, Repeatability, Stability, and Relative Accuracy for Fat-Soluble Vitamin-Related Compounds in Human Sera,” Anal. Chem. 69: 1406-1413 (1997).
Margolis, S.A. and Schapira, R.M. Liquid Chromatographic Measurement of L-Ascorbic Acid and D-Ascorbic Acid in Biological Samples, J. Chromatogr. B, 690: 25-33 (1997).
Sharpless, K.S., Brown Thomas, J., Turley, C.P., and Brewster, M.A., “Vitamin A,” In: LaboratoryMedicine: A Scientific and Managerial Infobase, Version 2.6, A.J.Pesce and L.A. Kaplan, (eds.) Pesce Kaplan Publisher, Cincinnati, OH, CD-Rom (1997).
Sharpless, K.S., Brown Thomas, J., Turley, C.P., and Brewster, M.A., “Vitamin E,” In: Laboratory Medicine: A Scientific and Managerial Infobase, Version 2.6, A.J.Pesce and L.A. Kaplan, (eds.) Pesce Kaplan Publisher, Cincinnati, OH, CD-Rom (1997).
Brown Thomas, J.; Kline, M. C.; Duewer, D. L.; Sharpless, K. E, The Stability of Retinol, α-Tocopherol, Trans-Lycopene, and Trans- β-Carotene in Liquid-frozen and Lyophilized Serum in Human Serum, Clin. Chim. Acta, 276:75-87 (1998).
Duewer, D. L.; Kline, M. C.; Sharpless, K. E.; Brown Thomas, J. M.; Gary, K. T.; Sowell, A. L. Micronutrients Measurement Quality Assurance Program: Helping Participants Use Inter-laboratory Comparison Exercise Results to Improve their Long-term Measurement Performance. Anal. Chem., 71: 1870 -1878 (1999).
Duewer. D.L., Kline, M.C., Sharpless, K.E., Brown Thomas, J., Stacewicz-Sapuntzakis, M., And Sowell, A.L., “NIST Micronutrients Measurement Quality Assurance Program: Characterizing Individual Participant Measurement Performance Over Time,” Anal. Chem., 72: 3611- 3619 (2000).
Duewer. D.L., Kline, M.C., Sharpless, K.E., and Brown Thomas, J., “NIST Micronutrients Measurement Quality Assurance Program: Characterizing the Measurement Community’s Performance Over Time,” Anal. Chem., 72: 4163 - 4170 (2000).
Brown Thomas, J., Kline, M.C., Gill, L.M., Yen, J.H., Duewer, D.L., Sniegoski, L.T., and Sharpless, K.E., “Preparation and Value Assignment of Constituents in Standard Reference Material 968c: Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum,” Clin. Chim. Acta, 305:141 -155 (2001).