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Development of Serum-Based SRMs to Assess Nutritional Status


A variety of nutrients are essential for normal functioning of the human body, and deficiencies can lead to serious illnesses. Accurate measurement of vitamin levels is therefore essential for identifying individuals affected by nutritional deficiencies and for evaluating the potential health benefits associated with dietary supplementation. Previous interlaboratory comparisons have indicated that discrepancies can exist between different assays used to assess nutritional status and that there is a significant need for standardization of these measurements. NIST is developing a series of serum-based SRMs that can be used as control materials by laboratories measuring vitamins (e.g., folate, D, B6, B12) in human serum.


Vitamin deficiencies are generally considered rare in the U.S., but a growing body of evidence suggests that certain deficiencies may be more prevalent than originally thought. For example, it has been estimated that 10% to 15% of adults over the age of sixty are affected by vitamin B12 deficiency, but most of these individuals lack any overt symptoms. Assessment of nutritional status has been performed by a variety of techniques, including microbiological, enzymatic, immunoassay, and chromatographic approaches. Interlaboratory comparisons have revealed discrepancies in the results obtained by laboratories using different assay methods or even when different laboratories use the same assay for a given analyte. Such variation adds significant complexity to the identification of “normal” ranges for nutritional analytes, and reduces the reliability of vitamin deficiency diagnoses. To improve the accuracy and reliability of nutritional status assessments, NIST is developing a series of serum-based Standard Reference Materials (SRMs).

Additional Technical Details:

SRM 1955 Homocysteine and Folate in Human Serum is currently available and consists of three sera with different analyte concentrations. SRM 968d Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum was released in 2008 and replaces SRM 968c. SRM 972 Vitamin D in Human Serum is currently in development and will consist of four different sera. Vitamin D status is generally monitored by measuring levels of 25-hydroxyvitamin D (25OHD). Because vitamin D has two forms, D2 and D3, accurate assessment of vitamin D status should include measurement of both hydroxylated forms (25OHD2 and 25OHD3). The levels of both analytes, as well as that of a related compound 3-epi-25OHD, will be characterized in the SRM.

SRM 3950 Vitamin B6 in Human Serum is another vitamin-related SRM that is currently in development and will consist of two sera with different analyte concentrations. Vitamin B6 is converted to pyridoxal 5'-phosphate (PLP), the analyte of clinical interest when assessing vitamin B6 dietary status. Although PLP deficiency is believed to be rare, tests for PLP deficiency are not widely performed because of a lack of accepted analytical methodology and because quality control materials are not readily available. SRM 3951 Vitamin B12 in Human Serum is the final serum-based SRM currently being developed and will consist of three sera with different analyte concentrations. Quantification of vitamin B12 in serum is particularly challenging because of the low levels present (pmol/L). Each of these SRMs has been designed in consultation with a panel of experts, and the analyte levels are intended to span the range typically found in patient samples.

Major Accomplishments:

  • SRMs for homocysteine and folate in serum (SRM 1955), as well as for fat-soluble vitamins and carotenoids in serum (SRM 968d), are currently available.
  • Candidate materials for SRM 972, SRM 3950, and SRM 3951 have been received, and value assignment is in progress
  • Higher-order methods have been developed or are in development for these vitamins

End Date:


Lead Organizational Unit:



Mary Bedner
Johanna E. Camara
Nathan G. Dodder
Stephen E. Long
Catherine A. Rimmer
Lorna T. Sniegoski
Susan S-C. Tai
Jeanice B. Thomas
Bryan E. Tomlin
Michael J. Welch

Johanna Camara
Phone 301-975-4672

Stephen A. Wise
Phone 301-975-3112