Analytical Threshold
1) The minimum height requirement at and above which detected peaks on a STR DNA profile electropherogram can be reliably distinguished from background noise; peaks above this threshold are generally not considered noise and are either artifacts or true alleles. 2) A 'Relative Fluorescence Units' (RFU) level determined to be appropriate for use in the PCR/STR DNA typing process; a minimum threshold for data comparison is identified by the specific forensic laboratory through independent validation studies.
Committee
Human Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 115 Standard for Training in Forensic Short Tandem Repeat Typing Methods using Amplification, DNA Separation, and Allele Detection. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2020.
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