Artifact
A non-allelic product of the amplification process (e.g., stutter, non-templated nucleotide addition, or other non-specific product), an anomaly of the detection process (e.g., pull-up or spike), or a byproduct of primer synthesis (e.g., 'dye blob') that may be observed on an electropherogram; some artifacts may complicate the interpretation of DNA profiles when they cannot be distinguished from the actual allele(s) from a particular sample.
Committee
Human Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 115 Standard for Training in Forensic Short Tandem Repeat Typing Methods using Amplification, DNA Separation, and Allele Detection. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2020.
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