Stochastic Effects

Changes in a DNA profile that generally occur when suboptimal or limiting quantities of DNA are tested. This may be due to sampling variation (e.g., pipetting) of the target DNA that goes into the polymerase chain reaction (PCR) and/or random events between primers and target DNA during PCR amplification. The effects may be observed at one or more loci, and include: 1) peak height imbalance of sister alleles in a heterozygous pair; 2) loss of data (referred to as 'allele drop out' when one or more alleles are missing at a locus and 'locus drop out' when all alleles are missing from a locus); 3) allele drop-in [allelic peak(s) in an electropherogram that are not reproducible]; and 4) elevated stutter peaks (a non-allelic peak in the stutter position exceeding the stutter expectation of the laboratory).