Light Sheet Fluorescence Microscopy (LSFM): Imaging Faster and Gentler

Light Sheet Fluorescence Microscopy (LSFM) has emerged as a powerful fluorescence microscopy tool for cell and developmental biology. LSFM is very well suited for imaging live samples because it offers optical sectioning, high-speed imaging, and low photobleaching and phototoxicity. During the course of the presentation, I will give a brief overview of the state of this field and then discuss our implementation of LSFM; a fiber coupled dual-view inverted Selected Plane Illumination Microscopy (diSPIM). diSPIM provides an isotropic resolution of 350 nm by computationally fusing two volumetric views acquired by switching illumination and detection between two perpendicular objectives.