Mari, F.
(1970),
In vivo testing of native α-conotoxins from the injected venom of Conus purpurascens, Biochemistry
(Accessed June 30, 2024)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
In vivo testing of native α-conotoxins from the injected venom of Conus purpurascens
Author(s)
Abstract
α-conotoxins selectively inhibit nicotinic acetylcholine receptors (nAChRs) and have been used as probes to study cholinergic pathways in vertebrates. Drosophila melanogaster expresses nAChRs in the CNS and is a suitable model to investigate the effects of alpha-conotoxins in vivo. Here we report the paired nanoinjection of native α-conotoxin PIA and two other novel α- conotoxins from the injected venom of Conus purpurascens and electrophysiological recordings of their effects on the giant fiber system (GFS) of D. melanogaster. αPIA, PIC and PIC[O7] caused differential disruption (αPIA > PIC > PIC[O7]) of the function of the giant fiber to dorsal longitudinal muscle (GF-DLM) pathway by inhibiting the Dα7 nAChR, a homolog to the vertebrate α7 nAChR.Citation
Biochemistry
Pub Type
Journals
Keywords
venom, cone snails, alpha-conotoxins, giant fiber system, Drosophila
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.
Created May 7, 2017, Updated January 27, 2020