Quantitative Methods to Characterize Cell Lines: Comparison of Cells from Marine and Terrestrial Mammals.

Published

July 28, 2012

Author(s)

Tighe Spurlin, John T. Elliott, Michael W. Halter, Kiran Bhadriraju, Alessandro Tona, Anne L. Plant, Annalaura Mancia, Bobby L. Middlebrooks, Gregory W. Warr

Abstract

Descriptive terms are often used to characterize cells in culture, but the use of nonquantitative and poorly defined terms can lead to ambiguities when comparing data from different laboratories. Although recently there has been a good deal of interest in unambiguous identification of cell lines via their genetic markers, it is also critical to have definitive, quantitative metrics to describe cell phenotypic characteristics. Quantitative metrics of cell phenotype will aid the comparison of data from experiments performed at different times and in different laboratories where influences such as the age of the population and differences in culture conditions or protocols can potentially affect cellular metabolic state and gene expression in the absence of changes in the genetic profile. Here, we present examples of robust methodologies for quantitatively assessing characteristics of cell morphology and cell-cell interactions, and of growth rates of cells within the population. We performed these analyses with endothelial cell lines derived from dolphin, bovine and human, and with a mouse fibroblast cell line. These metrics quantify some characteristics of these cells lines that clearly distinguish them from one another, and provide quantitative information on phenotypic changes in one of the cell lines over large number of passages.

Citation

Biotechnology Progress

Pub Type

Journals

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DOI Link

Citation

Spurlin, T. , Elliott, J. , Halter, M. , Bhadriraju, K. , Tona, A. , Plant, A. , Mancia, A. , Middlebrooks, B. and Warr, G. (2012), Quantitative Methods to Characterize Cell Lines: Comparison of Cells from Marine and Terrestrial Mammals., Biotechnology Progress, [online], https://doi.org/10.1002/btpr.1564 (Accessed November 27, 2024)