Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

Published

June 1, 2022

Author(s)

Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson

Abstract

Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8 buffer. Each component consists of one skirted 0.5-mL tube containing approximately 100 uL of DNA solution. Each tube is labeled and sealed with a removable screwcap. Here, we have documented the production, analytical methods, and statistical evaluation of realizing this RM.

Citation

Special Publication (NIST SP) - 260-225

Report Number

260-225

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.260-225

Local Download

Keywords

pathogen, microbial, DNA, metagenomics

Citation

Kralj, J. , Tourlousse, D. , Hunter, M. , Romsos, E. , Toman, B. , Vallone, P. and Jackson, S. (2022), Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-225, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933586 (Accessed November 21, 2024)