mDTEB, a new fluorescent label for carbohydrate nano-material in vivo studies

Published

May 27, 2023

Author(s)

Jeremiah Woodcock, Douglas M. Fox, Ilabahen A. Patel, Joy Dunkers, Stephan J. Stranick, Jeffrey W. Gilman

Abstract

Typical studies of gastral toxicity are conducted using radio labeling. This tends to be quite expensive and difficult owing to the required protocols for working with these materials and the expense of the chemical reagents. A possible alternative is fluorescence labeling. Fluorescence is just as sensitive as scintillation given that scintillation is itself a fluorescence measurement and subject to the same limitations. However, most fluorophores are sensitive to changes in pH and hydrolysis reactions present in most mammalian digestive tracts. Here we report the synthesis of a new pH insensitive and hydrolitically stable fluorophore, 10- (4-(3,5-dichlorophenoxy)phenyl)-2,8-diethyl-5,5-difluoro-1,3,7,9-tetramethyl-5H-4l4,5l4- dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinine (mDTEB). This fluorophore is based on the high quantum yield Boron-dipyrromethene (BODIPY) fluorescent center and is equipped with a reactive handle for convenient attachment to polysaccharides.

Citation

Cellulose

Volume

30

Pub Type

Journals

Download Paper

DOI Link

Keywords

Cellulose, fluorescence, BODIPY, fluorescence lifetime, pharmacokinetic studies

Citation

Woodcock, J. , Fox, D. , Patel, I. , Dunkers, J. , Stranick, S. and Gilman, J. (2023), mDTEB, a new fluorescent label for carbohydrate nano-material in vivo studies, Cellulose, [online], https://doi.org/10.1021/bc500004c (Accessed July 21, 2024)