Skip to main content
U.S. flag

An official website of the United States government

Determining the rates of \alpha-tocopherol movement in DPPC vesicles using Small Angle Neutron Scattering

Published

Author(s)

Dominik Dziura, Isabelle Dib, Omotayo Gbadamosi, Stuart R. Castillo, Maksymilian Dziura, Elizabeth Kelley, Drew Marquardt

Abstract

-Tocopherol (toc, vitamin E) is an essential nutrient sufficiently acquired through a balanced diet. This fat-soluble vitamin is most known for its antioxidative properties, however, its fundamental mechanism of action in cellular membranes remains unknown. To this end, we use time-resolved small angle neutron scattering (TR-SANS) and a contrast matching scheme to determine intervesicular exchange (kex) and intrabilayer flip-flop (kf) rates in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles. Moreover, we investigate the role of sample concentration and various types of cyclodextrins in affecting these rates. For the 25 mg/mL sample concentration it was determined that kex and kf were 1.35 ± 0.03 x 10-3 min-1 and 0.54 ± 0.10 x 10-3 min-1, which represent half-lives (T1/2) of 513.4 ± 11.7 and 1285.1 ± 242.7 minutes, respectively. Differential scanning calorimetry confirmed the observed timescales of toc movement.
Citation
Biophysical Journal

Keywords

tocopherol, vitamin E, DPPC, lipid, exchange, flip-flop, small angle neutron scattering

Citation

Dziura, D. , Dib, I. , Gbadamosi, O. , Castillo, S. , Dziura, M. , Kelley, E. and Marquardt, D. (2025), Determining the rates of \alpha-tocopherol movement in DPPC vesicles using Small Angle Neutron Scattering, Biophysical Journal (Accessed February 27, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created January 17, 2025, Updated January 22, 2025