Gallatin, G.
and Berglund, A.
(2012),
Optimal Laser Scan Path for Localizing a Fluorescent Particle in Two or Three Dimensions, Optics Express, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=910609
(Accessed December 13, 2024)
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Optimal Laser Scan Path for Localizing a Fluorescent Particle in Two or Three Dimensions
Published
Author(s)
,
Abstract
Localizing a fluorescent particle by scanning a focused laser in its vicinity and analyzing the detected photon stream provides real-time information for a modern class of feedback control systems for particle tracking and trapping. We present a simple mathematical test to determine whether a particular laser scan path encodes maximal information about a fluorescent particle's position in the detected photon stream. We give the optimal path for a Gaussian laser scanned in two or three dimensions, and present the Cram\'{e}r-Rao bound on the two and three-dimensional localization accuracy that can be achieved by analyzing the detected photon stream.Citation
Optics Express
Volume
20
Issue
15
Pub Type
Journals
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Keywords
single particle tracking
Citation
Issues
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Created July 16, 2012, Updated February 19, 2017