Towards optimization and characterization of dye-embedded gold nanoclusters for multiplexed optical imaging

Published

February 26, 2009

Author(s)

Michael A. McDonald, Angela R. Hight Walker

Abstract

Metallic nanoparticle aggregates coupling strong surface plasmons with a Raman reporter molecule have been developed for application in multiplexed optical imaging. Of interest to our work is the ability of the agents to serve as surface-enhanced Raman spectroscopy (SERS) probes. We present the seed-mediated synthesis and characterization of rhodamine B isothiocyante Au nanoclusters (RhB-AuNCs). RhB-AuNCs are anisotropic structures which contain the Raman reporter, RhB, embedded between a gold aggregate core and gold surface layer. In contrast to typical SERS studies, the Raman signal originates from the probe (RhB-AuNCs) and not from RhB incubated with a noble metal colloid. Characterization of the probes optical properties is presented. The overall goal of our study is to prepare probes that may be used for the identification and spectroscopic labeling of multiple molecular biomarkers utilizing SERS imaging.

Proceedings Title

Plasmonics in Biology and Medicine VI
Proceedings of SPIE Volume 7192

Volume

7192

Conference Dates

January 24-29, 2009

Conference Location

San Jose, CA

Conference Title

Conference 7192 - BIOS 2009/Photonics West

Pub Type

Conferences

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Keywords

gold, nanoparticle, plasmonics, Raman, SERS

Citation

McDonald, M. and Hight, A. (2009), Towards optimization and characterization of dye-embedded gold nanoclusters for multiplexed optical imaging, Plasmonics in Biology and Medicine VI Proceedings of SPIE Volume 7192, San Jose, CA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=901384 (Accessed January 2, 2025)