Rapid Screening of Hydrogel Properties for 3D Tissue Culture: Effect of Modulus on Encapsulated Osteoblasts

Published

April 25, 2009

Author(s)

Kaushik Chatterjee, Sheng Lin-Gibson, Marian F. Young, William E. Wallace, Carl Simon Jr.

Abstract

Statement of Purpose: With few successful products in the market despite large investments in tissue engineering research, there is a need to accelerate tissue engineering research. Towards this objective, developing high-throughput platforms to rapidly screen cell-biomaterial interactions offers a plausible solution. Furthermore, cells cultured in 3-dimensions (3D) behave more physiologically than those cultured on 2-dimensional (2D) surfaces1. Therefore, in this work cell-material interactions were studied using combinatorial methods where cells are cultured in 3D. Polyethylene glycol (PEG)-based hydrogels have emerged as promising tissue engineering scaffolds in recent years2. It is well-reported that modulus of underlying substrate influences cell behavior3. The objective of this work was to examine the effect of mechanical properties (compressive modulus) on osteoblasts encapsulated within gradients of hydrogel modulus. Preliminary data presented herein indicate that differentiation and proliferation of encapsulated mouse osteoblasts are profoundly influenced by the stiffness of the hydrogel.

Proceedings Title

Society for Biomaterials Annual Meeting

Conference Dates

April 22-25, 2009

Conference Location

San Antonio, TX, US

Pub Type

Conferences

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Keywords

hydrogel, poly(ethylene glycol) dimethacrylate, osteoblast, modulus, combinatorial methods, cell differentiation

Citation

Chatterjee, K. , Lin-Gibson, S. , Young, M. , Wallace, W. and Simon Jr., C. (2009), Rapid Screening of Hydrogel Properties for 3D Tissue Culture: Effect of Modulus on Encapsulated Osteoblasts, Society for Biomaterials Annual Meeting, San Antonio, TX, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=900245 (Accessed October 18, 2025)