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Kirsten Parratt (Fed)
Biomedical Engineer
OVERVIEW
Kirsten Parratt, PhD is a Biomedical Engineer in the Biomaterials Group.
Microbial cell characterization work: Kirsten leads flow cytometry efforts related to the quantitation and characterization of microbial cells in support of Rapid Microbial Testing Methods for advanced therapy products and whole cell microbial reference material development (including the new Whole Gut Microbiome RM).
Data and metadata work: Kirsten co-leads projects on data standards development (ASTM WK83732) and research data management (electronic lab notebook, data repository) for Microbial Metrology. She is also a member of the ISAC Data Committee with a focus on Standards and Metadata.
Previous work: Kirsten was a NIST-NRC Postdoctoral Research Associate mentored by Nancy J. Lin. The goal of her NRC work was to develop quantitative measurements of adherent microbial communities and biofilm-material interactions. Her graduate dissertation research under Krish Roy focused on using flow cytometry as a high-throughput platform to assay microfabricated biomaterials for stem cell differentiation studies and detection applications.
PUBLICATIONS
- Servetas, SL., Parratt, KH., Brinkman, NE., Shanks, OC., Smith, T., Mattson, PJ., Lin, NJ. “Standards to support an enduring capability in wastewater surveillance for public health: Where are we?” Case Studies in Chemical and Environmental Engineering (2022). https://www.sciencedirect.com/science/article/pii/S266601642200069X
- Inwood, SL., Tian, L., Parratt, K., Maragh, S., Wang, L. “Evaluation protocol for CRISPR/Cas9-mediated CD19 knockout GM24385 cells by flow cytometry and Sanger sequencing” Biotechniques (2022). https://www.future-science.com/doi/full/10.2144/btn-2022-0015
- Lin, NJ., Servetas, SL., Jackson, SA., Lippa, KA., Parratt, K., Mattson, P., Beahn, C., Mattioli, M., Gutierrez, S., Focazio, M., Smith, T., Storella, P., Wright, S. “Report on the DHS/NIST Workshop on Standards for an Enduring Capability in Wastewater Surveillance for Public Health” (2022). https://nvlpubs.nist.gov/nistpubs/SpecialPublications/NIST.SP.1279.pdf
- Lin, NJ., Jackson, SA., Servetas, S., Parratt, K., Dunkers, J., Eskandari, T., Lin-Gibson, S. “Report from the NIST Workshop: Launch of the Rapid Microbial Testing Methods Consortium” (2022). https://nvlpubs.nist.gov/nistpubs/SpecialPublications/NIST.SP.1272.pdf
- Parratt, K.; Jeong, J.; Qiu, P.; Roy, K. 3D Material Cytometry (3DMaC): A Very High-replicate, High-throughput Analytical Method using Microfabricated, Shape-specific, Cell-material Niches. Lab on a Chip, 2017, 17, 2861-2872.
- Parratt, K.; Smerchansky, M.; Stiggers, Q.; Roy, K. Effect of Hydrogel Material Composition on hBMSC Differentiation into Zone-specific Neo-cartilage: Engineering Human Articular Cartilage with Spatially Varying Properties. Journal of Materials Chemistry B, 2017, 5, 6237-6248.
- Dwarshuis, N.; Parratt, K.; Santiago-Miranda, A.; Roy, K. Cells as Advanced Therapeutics: State-of-the-art, Challenges, and Opportunities in Large Scale Biomanufacturing of High-Quality Cells for Adoptive Immunotherapies. Advanced Drug Delivery Reviews, 2017, 114, 222-239.