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Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

Published

Author(s)

Tracey Schock, Christina Jones, Federico Torta, Nils Hoffmann, Bo Burla, Irina Alecu, Makoto Arita, Takeshi Bamba, Steffany A.L. Bennett, Justine BERTRAND-MICHEL, Britta Brugger, Mónica Molina, María Dolores Camacho Muñoz, Antonio Checa, Michael Chen, Michelle Cinel, Benoit COLSCH, Cristina Coman, Bebiana Da Costa Sousa, Alex Dickens, Maria Maria, Finnur Freyr Eiríksson, Hector Gallart-Ayala, Mohan Ghorasaini, Martin Giera, Xue Guan, Mark Haid, Thomas Hankemeier, Amy Harms, Marcus Höring, Michal Holčapek, Thorsten Hornemann, Chunxiu Hu, Andreas Hülsmeier, Julijana Ivanisevic, Harald Köfeler, Sin Man Lam, Jong Cheol Lee, Gerhard Liebisch, Andrea F Lopez-Clavijo, Malena Manzi, Peter Meikle, Maria Monge, Sneha Muralidharan, Anna Nicolaou, Valerie O'Donnell, Matej Oresic, Arvind Ramanathan, Daisuke Saigusa, Heidi Schwartz-Zimmermann, Guanghou Shui, Masatomo Takahashi, Margrét Thorsteinsdóttir, Anthony Tournadre, Hiroshi Tsugawa, Victoria Tyrrell, Grace van der Gugten, Michael Wakelam, Craig Wheelock, Denise Wolrab, Guowang Xu, John Bowden, Kim Ekroos, Robert Ahrends, Markus Wenk

Abstract

In this community effort, we compared measurements between 34 laboratories from 19 countries, utilizing mixtures of authentic synthetic standards, to quantify by mass spectrometry four ceramide species in the NIST human blood plasma Standard Reference Material (SRM) 1950, as well as three other NIST plasma Reference Materials (RM 8231). Participants utilized a validated method and/or methods of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variations (CV) were calculated using single-point and multi-point calibrations, respectively. In SRM 1950 these concentration values are 0.1206 ± 0.0038 µmol/L for Cer 18:1;O2/16:0 (n=36), 0.0412± 0.0012 µmol/L for Cer 18:1;O2/18:0 (n=31), 1.1898 ± 0.0229 µmol/L for Cer 18:1;O2/24:0 (n=35) and 0.4293 ± 0.0064 µmol/L Cer 18:1;O2/24:1 (n=27, mean ± SEM, based on calibration curve quantification). Our results are the most precise (intra-laboratory CV ≤ 4.5%) and concordant (inter-laboratory CV < 19%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. Furthermore, we demonstrate that calibration using authentic standards dramatically reduces data variability originating from different datasets utilizing either triple quadrupole-based (n=32) or high resolution (n=6) mass analyzers with different methods (e.g., an inter-lab CV of 11% for Cer 18:1;O2/24:0 when quantified by authentic Cer 18:1;O2[d7]/24:0 standard vs. 60% when the shorter chain Cer 18:1;O2[d7]/16:0 standard is used). Collectively, the results from this study provide a rich experience base for translation of lipidomic technologies to clinical applications such as the determination of reference intervals (RIs) in human populations or reference change values (RCV), in which case analytical variability is a key factor for recall during multiple testing of individuals.
Citation
Nature Communications

Keywords

ceramides, harmonization, lipidomics, mass spectrometry, NIST, reference material

Citation

Schock, T. , Jones, C. , Torta, F. , Hoffmann, N. , Burla, B. , Alecu, I. , Arita, M. , Bamba, T. , Bennett, S. , BERTRAND-MICHEL, J. , Brugger, B. , Molina, M. , Camacho Muñoz, M. , Checa, A. , Chen, M. , Cinel, M. , COLSCH, B. , Coman, C. , Da Costa Sousa, B. , Dickens, A. , Maria, M. , Eiríksson, F. , Gallart-Ayala, H. , Ghorasaini, M. , Giera, M. , Guan, X. , Haid, M. , Hankemeier, T. , Harms, A. , Höring, M. , Holčapek, M. , Hornemann, T. , Hu, C. , Hülsmeier, A. , Ivanisevic, J. , Köfeler, H. , Lam, S. , Lee, J. , Liebisch, G. , Lopez-Clavijo, A. , Manzi, M. , Meikle, P. , Monge, M. , Muralidharan, S. , Nicolaou, A. , O'Donnell, V. , Oresic, M. , Ramanathan, A. , Saigusa, D. , Schwartz-Zimmermann, H. , Shui, G. , Takahashi, M. , Thorsteinsdóttir, M. , Tournadre, A. , Tsugawa, H. , Tyrrell, V. , van der Gugten, G. , Wakelam, M. , Wheelock, C. , Wolrab, D. , Xu, G. , Bowden, J. , Ekroos, K. , Ahrends, R. and Wenk, M. (2024), Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards, Nature Communications, [online], https://doi.org/10.1038/s41467-024-52087-x, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=956339 (Accessed November 21, 2024)

Issues

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Created October 3, 2024, Updated October 30, 2024