Cleveland, M.
, He, H.
, Milavec, M.
, Bae, Y.
, Vallone, P.
and Huggett, J.
(2024),
Digital PCR for the Characterization of Reference Materials, Molecular Aspects of Medicine, [online], https://doi.org/10.1016/j.mam.2024.101256, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=956877
(Accessed October 31, 2024)
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Digital PCR for the Characterization of Reference Materials
Published
Author(s)
, , Mojca Milavec, Young-Kyung Bae, , Jim Huggett
Abstract
Well characterized reference materials are essential to ensuring the harmonization and accuracy of nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well its role when developing and characterizing nucleic acid-based reference materials.Citation
Molecular Aspects of Medicine
Pub Type
Journals
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Keywords
digital PCR, dPCR, reference materials, molecular diagnostics
Citation
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Created February 14, 2024, Updated February 20, 2024