Zangmeister, R.
and Tarlov, M.
(2004),
DNA Displacement Assay Integrated into Microfluidic Channels, Analytical Chemistry
(Accessed March 14, 2025)
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DNA Displacement Assay Integrated into Microfluidic Channels
Published
Author(s)
,
Abstract
This paper describes the development of a unique fluorescence based DNA diagnostic assay incorporated into a microfluidic channel that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through a hydrogel plug sensing platform immobilized in microfluidic channels. The distinct advantages of this assay are the short sensing times, as a result of directed electrophoretic transport of target DNA to the sensing element, combined with the ability to detect non-labeled target DNA.Citation
Analytical Chemistry
Volume
76
Issue
No. 13
Pub Type
Journals
Keywords
Displacement assay, DNA sensing, Microfluidics, Polyacrylamide hydrogel
Citation
Issues
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Created July 1, 2004, Updated February 17, 2017