Handheld Real-Time PCR Device

Published

January 26, 2016

Author(s)

Christian D. Ahrberg, Robert Ilic, Andreas Manz, Pavel Neu?il

Abstract

Here we report one of the smallest real-time PCR system up to date with approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in form of virtual reaction chambers (VRC) where a ≈ 100 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate PCR performance. The standard curve slope was (-3.02 ± 0.16) CT /dec (mean ± standard deviation) corresponding to an amplification efficiency of (0.91 ± 0.05) per cycle (mean ± standard deviation). The PCR device was capable of detecting a single DNA copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.

Citation

Lab on A Chip

Volume

16

Issue

3

Pub Type

Journals

Download Paper

https://doi.org/10.1039/C5LC01415H

Local Download

Keywords

real-time PCR, hand held, portable, standard curve

Citation

Ahrberg, C. , Ilic, R. , Manz, A. and Neu?il, P. (2016), Handheld Real-Time PCR Device, Lab on A Chip, [online], https://doi.org/10.1039/C5LC01415H, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=919664 (Accessed December 26, 2024)