An Instrumented Bioreactor for Cartilage Tissue Engineering

Published

April 29, 2009

Author(s)

Timothy P. Quinn, Colm Flannery, Damian S. Lauria, Douglas V. Gallagher, Kristi S. Anseth

Abstract

Bioreactors for in vitro growth of engineered tissue (ET) would ideally include sensors that would continuously measure the functional quality of the construct during incubation. Principles of feedback control could then be used to optimize properties of the ET. The bioreactor presented here is capable of measuring force during compressive mechanical stimulation, on line mechanical characterization (stress-strain) of the construct, optical inspection, and integrated ultrasonic imaging for determining extracellular matrix content. The design allows for mechanical property measurements in a range appropriate for the hydrogel constructs that will be used in the bioreactor. The response of the system allows for mechanical stimulation waveforms with high-fidelity tracking (within the resolution of the actuator) at least up to 10 Hz. The ultrasonic sensing system gave a significant correlation between the attenuated reflection coefficient and collagen content in degraded bovine samples.

Proceedings Title

Proceedings of the 2009 Annual Meeting and Exposition of the Society of Biomaterials

Conference Dates

April 22-25, 2009

Conference Location

San Antonio, TX

Conference Title

The 2009 Annual Meeting and Exposition of the Society of Biomaterials

Pub Type

Conferences

Keywords

Cartilage, Tissue Engineering, Online Sensing, Bioreactor, Materials Testing, Ultrasound

Citation

Quinn, T. , Flannery, C. , Lauria, D. , Gallagher, D. and Anseth, K. (2009), An Instrumented Bioreactor for Cartilage Tissue Engineering, Proceedings of the 2009 Annual Meeting and Exposition of the Society of Biomaterials, San Antonio, TX (Accessed December 21, 2024)