Ross, D.
, Tonner, P.
and Vasilyeva, O.
(2022),
Method for reproducible automated bacterial cell culture and measurement, Synthetic Biology, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933612
(Accessed November 20, 2024)
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Method for reproducible automated bacterial cell culture and measurement
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Abstract
Microbial cell culture is one of the most commonly performed protocols for synthetic biology, and laboratories are increasingly using 96-well plates and laboratory automation systems for cell culture. Here we describe a method for reproducible microbial culture using laboratory automation systems including automated liquid handlings, automated plate sealing and de-sealing, and automated incubation and measurement of growing cultures. We discuss the key considerations that, in our experience, are important for reproducibility, and present statistical analyses of data from 150 automated microbial growth experiments performed over 27 months using our automated method.Citation
Synthetic Biology
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Journals
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Keywords
Laboratory Automation, Synthetic Biology, Cell Culture
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Created August 8, 2022, Updated October 23, 2024