Nondestructive, Longitudinal, 3D Cell Viability Assessment in a Multi-well Plate System Using Electron Paramagnetic Resonance Oxygen Imaging

Published

May 1, 2024

Author(s)

Safa Hameed, Navin Viswakarma, Eliyas Siddiqui, Greta Babakhanova, Carl Simon Jr., Boris Epel, Mrignayani Kotecha

Abstract

Cell viability is an essential measurement for cell therapy, tissue engineered medical products (TEMPs), drug development, and many other biological processes and products. These systems rely on viable, healthy, and functional cells to work as intended. However, commonly used methods to measure cell viability are destructive and do not provide temporospatial information. The use of oxygen by cells and tissues is an essential aspect of metabolism and a reliable indicator of viability. Here, we report 3D partial oxygen pressure (pO2) mapping of live cells over time as a measure of viability. For pO2 imaging, we utilized trityl OX071-based pulse electron paramagnetic resonance oxygen imaging (EPROI). We developed a novel incubator-resonator platform to perform pO2 imaging of live cells in 96-well plates during planar 2D culture and during culture in a 3D hydrogel scaffolds. This is the first study showing the nondestructive cell viability assessment using 3D oxygen imaging and it may impact cell therapy, tissue engineering, regenerative medicine, and other fields.

Citation

Science Advances

Volume

2

Pub Type

Journals

Download Paper

https://doi.org/10.1038/s44303-024-00013-7

Local Download

Keywords

cell viability, tissue engineering, regenerative medicine, hydrogel, scaffold, imaging

Citation

Hameed, S. , Viswakarma, N. , Siddiqui, E. , Babakhanova, G. , Simon Jr., C. , Epel, B. and Kotecha, M. (2024), Nondestructive, Longitudinal, 3D Cell Viability Assessment in a Multi-well Plate System Using Electron Paramagnetic Resonance Oxygen Imaging, Science Advances, [online], https://doi.org/10.1038/s44303-024-00013-7, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=936271 (Accessed September 26, 2024)