Preparation, Characterization, and Biological Activity of Stability-Enhanced Polyethyleneimine-Conjugated Gold Nanoparticles (Au-PEI@NIST) for Biological Application

Published

September 16, 2021

Author(s)

Tae Joon Cho, Vincent A. Hackley, Feng Yi, David A. LaVan, Vytas Reipa, Alessandro Tona, Bryant C. Nelson, Christopher Sims, Natalia Farkas

Abstract

In this special publication, we report on a modified synthetic process that results in a stable suspension of Au-PEI nanoparticles, Au-PEI@NIST. Synthesized materials were characterized using an orthogonal approach that included dynamic light scattering (DLS), UV-Vis absorbance (UV-Vis), transmission electron microscopy (TEM), atomic force microscope (AFM), and thermogravimetric analysis (TGA). Very importantly, the end-product in this study demonstrated excellent colloidal stability in multiple aqueous environments including over a wide pH and temperature range of physiological relevance. The product also exhibited long-term storage stability under ambient conditions. Furthermore, the cytotoxicity of Au-PEI@NIST was investigated using the CHO K1 cell line and exhibited dose proportional toxicity, which suggested potential for use as a positive control in nanotoxicology studies. The results and stringent testing regimen point toward an effective and sustainable approach that can serve as a clarified starting point for the development of more complex conjugates for nanomedicine area.

Citation

Special Publication (NIST SP) - 1200-29

Report Number

1200-29

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.1200-29

Local Download

Keywords

AuNPs, Au-PEI, gold nanoparticles, gold-polyethyleneimine, polyethyleneimine, positively charged gold nanoparticles, colloidal stability, nanotoxicity.

Citation

Cho, T. , Hackley, V. , Yi, F. , LaVan, D. , Reipa, V. , Tona, A. , Nelson, B. , Sims, C. and Farkas, N. (2021), Preparation, Characterization, and Biological Activity of Stability-Enhanced Polyethyleneimine-Conjugated Gold Nanoparticles (Au-PEI@NIST) for Biological Application, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.1200-29, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933147 (Accessed November 23, 2024)