Henrickson, S.
, DiMarzio, E.
, Wang, Q.
, Stanford, V.
and Kasianowicz, J.
(2010),
Probing Single Nanometer-Scale Pores with Polymeric Molecular Rulers, Journal of Chemical Physics, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=32215
(Accessed December 22, 2024)
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Probing Single Nanometer-Scale Pores with Polymeric Molecular Rulers
Published
Author(s)
Sarah E. Henrickson, Edmund A. DiMarzio, Qian Wang, ,
Abstract
It has been shown that individual molecules of single stranded DNA can be driven electrophoretically through a single Staphylococcus aureus ?-hemolysin ion channel. Polynucleotides thread through the channel as extended chains and the polymer-induced ionic current blockades exhibit stable modes during the interactions. Additionally, we show here that polynucleotides can be used to probe structural features of the ?-hemolysin channel itself. Specifically, both pore length and channel aperture profile can be estimated. The results are consistent with the channel crystal structure and suggest that polymer-based ?molecular rulers? may prove useful in deducing the structures of nanometer- scale pores in general.Citation
Journal of Chemical Physics
Volume
132
Pub Type
Journals
Download Paper
Keywords
alpha hemolysin, alpha toxin, DNA, ion channel, nanopore, polymer confinement, polynucleotide, structure-function
Citation
Issues
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Created April 1, 2010, Updated October 12, 2021