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PUBLICATIONS

Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation

Published

Author(s)

Patricia Kiesler, Samantha Maragh, Stella Lee, Alexis Norris, Mayumi Miller, Adam Moyer

Abstract

Genome editing technology is being used in animals for a variety of purposes, including improvement of animal and public health outcomes. Characterization of genome editing reagents and anticipated genomic alterations is an essential step toward the development of an edited animal. Here, we present a protocol for genome editing in the swine testicular (ST) cell line. We describe steps for evaluating CRISPR-Cas9 complex functionality in vitro, delivering editing molecules into cells by transfection, and assessing target editing via Sanger sequencing.
Citation
STAR Protocols
Volume
5
Issue
4
Pub Type
Journals

Download Paper

DOI Link

Keywords

CRISPR, transfection, cell culture
Cell measurements and Bioscience

Citation

Kiesler, P. , Maragh, S. , Lee, S. , Norris, A. , Miller, M. and Moyer, A. (2024), Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation, STAR Protocols, [online], https://doi.org/10.1016/j.xpro.2024.103385 (Accessed March 13, 2025)

Issues

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Created December 20, 2024, Updated March 6, 2025