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Displaying 301 - 325 of 482

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

Genomic DNA of Nostoc Commune (Cyanobacteria) Becomes Covalently Modified During Long-Term (Decades) Desiccation But Is Protected From Oxidative Damage and Degradation

June 15, 2003
Author(s)
B Shirkey, N J. McMaster, Sue C. Smith, D J. Wright, H Rodriguez, Pawel Jaruga, M Birincioglu, R F. Helm, M Potts
Genomic DNA of Nostoc commume (Cyanobacteria) became covalently modified during decades of desiccation. Amplification of gene loci from desiccated cells required pretreatment of DNA with N-phenacylthiazolium bromide; a reagent that cleaves DNA- and protein

Specific Antibody-DNA Interaction: A Novel Strategy for Tight DNA Recognition

May 27, 2003
Author(s)
S M. Di Pietro, J M. Centeno, M L. Cerutti, M F. Lodeiro, D U. Ferreiro, L G. Alonso, Frederick P. Schwarz, F Goldbaum, G de Prat-Gay
Taking advantage of the extreme stability of a protein-DNA complex, we obtained highly specific monoclonal antibodies against a predetermined palindromic double stranded DNA sequence, corresponding to the binding site of the E2 transcriptional regulator of

Arabidopsis Thaliana Ogg1 Protein Excises 8-Hydroxyguanine and 2,6- Diamino-4-hydroxy-5-formamidopyrimidine from Oxidative Damaged DNA Containing Multiple Lesions

March 18, 2003
Author(s)
T Morales-Ruiz, M Birincioglu, Pawel Jaruga, H Rodriguez, T. Roldan-Arjona, Miral M. Dizdar
A functional homologue of eukaryotic Ogg1 proteins in the model plant Arabidopsis thaliana has recently been cloned, isolated, and characterized [Garcia-Ortiz, M.V., Ariza, R.R., and Roldan-Arjona, T. (2001) Plant Mol. Biol. 47, 795-804]. This enzyme

DNA-Based Sensors

January 1, 2003
Author(s)
Michael J. Tarlov, A B. Steel
The development of DNA-based sensors for genetic assay and sequencing applications has exploded over the last decade. Nearly all of these devices rely on the hybridization of a complementary oligonucleotide with surface-confined, single-stranded DNA or RNA

Oxidative DNA Damage Biomarkers Used in Tissue Engineered Skin

January 1, 2003
Author(s)
H Rodriguez, Pawel Jaruga, M Birincioglu, Peter E. Barker, C D. O'Connell, M. Dizdaroglu
To assure that tissue engineered medical products are free of genetic changes that might occur from inflammation during the development phase of the product, our laboratory is responding to the need for test methods used to assess the safety and
Displaying 301 - 325 of 482