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Displaying 601 - 625 of 838

Metrology and Standards Needs of Some Categories of Medical Devices

April 30, 2008
Author(s)
J C. Chiao, Julian M. Goldman, David A. Heck, Peter Kazanzides, William J. Peine, James B. Stiehl, Dwight Yen, Nicholas Dagalakis
With rapid advances in meso, micro and nano scale technology devices and electronics a new generation of advance medical devices is emerging, which promises less invasive and more accurate, automated and effective medical treatments. We examined the

Commutability of NIST SRM 1955 Homocysteine and Folate in Frozen Human Serum With Selected Total Homocysteine Immunometric or Enzymatic Assays

March 1, 2008
Author(s)
B C. Nelson, Christine M. Pfeiffer, Ming Zhang, David L. Duewer, Katherine E. Sharpless, Katrice Lippa
Background: The National Institute of Standards and Technology (NIST) and the Centers for Disease Control and Prevention (CDC) have recently developed Standard Reference Material (SRM) 1955 Homocysteine and Folate in Frozen Human Serum with certified

Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

January 25, 2008
Author(s)
Carolyn R. Steffen, Margaret C. Kline, Michael Coble, John M. Butler
An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized to aid in the analysis of degraded DNA samples. These new markers produce short PCR products in the target range of 50 150 base pairs (bp) by moving the

Toward Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

December 28, 2007
Author(s)
Lili Wang, Adolfas Gaigalas, Gerald Marti, Fatima Abbasi, Robert A. Hoffman
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra

Epitope Selection of Monoclonal Antibodies for Interleukin-4 Quantification Using Suspension Arrays and Forward-Phase Protein Microarrays

December 6, 2007
Author(s)
Lili Wang, Kenneth D. Cole, Hua-Jun He, Alexander W. Peterson, Adolfas K. Gaigalas, Y Zong
A recombinant mouse interleukin-4 (IL-4) and three different purified rat anti-mouse IL-4 monoclonal antibodies (Mab) with different epitopes were employed as a model system. This system was used to examine monoclonal antibody effectiveness using both

Thermodynamics of Enzyme Catalyzed Reactions: Part 7 - 2007 Update

October 25, 2007
Author(s)
Robert N. Goldberg, Yadu D. Tewari, Talapady N. Bhat
This review serves to update previously published evaluations of equilibrium constants and enthalpy changes for enzyme catalyzed reactions. For each reaction the following information is given: the reference for the data; the reaction studied; the name of

Covalent Attachment of Photoluminescent Silicon Nanoparticles to Streptavidin

September 11, 2007
Author(s)
Jonghoon Choi, Peter Niarhos, Nam S. Wang, Vytas Reipa
We have covalently attached multiple fluorescent silicon nanocrystals (SNs) to streptavidin molecule. Selective conjugation of SNs to a target protein is accomplished using sequential silicon surface termination chemistry. In the first step, freshly

Characterization of degradation mechanisms in neural recording electrodes

August 1, 2007
Author(s)
Jennifer Anton, Stephanie A. Hooker
Understanding the nature of specific neural activity is essential to the progression of research in the field of brain disorders and diseases, as well as neuroprosthetics. Microelectrodes are the primary measurement devices used to transduce neural

Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits

July 25, 2007
Author(s)
Carolyn R. Steffen, Margaret C. Kline, Julio J. Mulero, Robert E. Lagace, Chien-Wei Chang, Lori K. Hennessy, John M. Butler
The AmpFlSTR MiniFiler PCR Amplification kit developed by Applied Biosystems enables size reduction on eight of the larger short tandem repeat (STR) loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA

Recent Developments of the HLPR Chair

June 15, 2007
Author(s)
Roger V. Bostelman, James S. Albus, Tommy Chang
The Home Lift, Position and Rehabilitation (HLPR) Chair, developed at the National Institute of Standards and Technology (NIST) within the Intelligent Systems Division (ISD) from 2005 to 2006, is currently being evaluated for static stability using

Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes

May 25, 2007
Author(s)
Dustin Levy, Kimberly Briggman
The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder

A new model of pulse oximetry: Two-dimensional pulsation

March 14, 2007
Author(s)
Shao Yang, Paul B. Batchelder, Dena M. Raley
We developed a two-dimensional pulsation model of pulse oximetry. Instead of a one-dimensional single layer as in the conventional theory, the arteries pulsate in two dimensions taking into account the effect of probing light that does not pass through the

The Stiffness of Collagen Fibrils Influences Vascular Smooth Muscle Cell Phenotype

March 1, 2007
Author(s)
Dennis P. McDaniel, Gordon A. Shaw, John T. Elliott, Kiran Bhadriraju, Curtis W. Meuse, Koo-Hyun Chung, Anne L. Plant
Cells receive signals from the extracellular matrix through receptor-dependent interactions, but they are also influenced by the mechanical properties of the matrix. While bulk properties of substrates have been shown to effect cell behavior, we show here

Counter-Flow Gradient Electrofocusing

February 1, 2007
Author(s)
Jonathan G. Shackman, David J. Ross
Counter-flow gradient electrofocusing methods are methods whereby a combination of electrophoresis and a bulk solution counter-flow is used to accumulate or focus analytes at stationary points along a separation column. This review first describes the

Characterization and Control of Lipid Layer Fluidity in Hybrid Bilayer Membranes

January 31, 2007
Author(s)
Neil A. Anderson, Lee J. Richter, John C. Stephenson, Kimberly A. Briggman
The main gel-to-liquid-crystal (LC) phase transition temperature, Tm, of the distal lipid layer in hybrid bilayer membranes (HBMs) under water was investigated using vibrational sum frequency spectroscopy (VSFS). VSFS has unique sensitivity to order
Displaying 601 - 625 of 838