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Displaying 51 - 75 of 160

The Metrology of Counting Protein Particles

July 13, 2013
Author(s)
Dean C. Ripple, Michael J. Carrier, Richard E. Cavicchi, Christopher B. Montgomery, Zhishang Hu
A common degradation pathway for protein-based drugs is the growth of protein aggregates or particles. Counting and characterization of these particles is needed to assure the quality, efficacy, and safety of this type of drug. The unusual physical

The Metrology of Counting Protein Particles

March 18, 2013
Author(s)
Dean C. Ripple, Michael J. Carrier, Richard E. Cavicchi, Christopher B. Montgomery, Zhishang Hu
A common degradation pathway for protein-based drugs is the growth of protein aggregates or particles. Counting and characterization of these particles is needed to assure the quality, efficacy, and safety of this type of drug. The unusual physical

Uncertainty Budget for the NIST Hybrid Humidity Generator

December 1, 2012
Author(s)
Christopher W. Meyer, W Wyatt Miller, Dean C. Ripple, Gregory E. Scace
We provide here a detailed uncertainty budget for the new Hybrid Humidity Generator (HHG) that has been constructed at the National Institute of Standards and Technology (NIST). The HHG generates frost/dew points from –70 °C to +85 °C using calibration gas

A Humidity Generator for Temperatures to 200 degC and Pressures to 1.6 MPa

November 30, 2012
Author(s)
D Vega-Maza, W. Wyatt Miller, Dean C. Ripple, Gregory E. Scace
We have constructed a new humidity generator that produces gas streams of known moisture content at temperatures from 85 °C to 200 °C, absolute pressures from 0.2 MPa to 1.6 MPa, and relative humidities from 10 % to 90 %. The generator produces a moist gas

Room Temperature Acoustic Transducers for High-Temperature Thermometry

July 9, 2012
Author(s)
Dean C. Ripple, William E. Murdock, Gregory F. Strouse, Keith A. Gillis, Michael R. Moldover
We have successfully conducted highly-accurate, primary acoustic thermometry at 600 K using a sound source and a sound detector at room temperature. We describe the source, the detector, and the ducts that connected them to our cavity resonator. This

Protein Particles: What We Know and What We Dont Know

June 26, 2012
Author(s)
Dean C. Ripple, Mariana Dimitrova
All therapeutic protein products contain particles formed by the aggregation of protein monomers. There is a growing interest in the understanding of particles in biopharmaceutical products, which has been fostered on the one hand by significant

Standards for the Optical Detection of Protein Particulates

January 23, 2012
Author(s)
Dean C. Ripple, Michael J. Carrier, Joshua R. Wayment
Particulates in protein therapeutics, composed of agglomerated protein monomers, may cause an immunogenic response in patients. Consequently, industry and the FDA desire more accurate methods for counting and characterizing particulates. Unlike likely

The Residual Resistance Ratio of High-Purity Platinum

August 22, 2011
Author(s)
Weston L. Tew, William E. Murdock, Michal J. Chojnacky, Dean C. Ripple
High-purity platinum wire is produced for use in both Standard Platinum Resistance Thermometers (SPRTs) and type S and type R thermocouples. The resistance ratio W(T90) and thermoelectric characteristics of the wire are a function of chemical purity and

The Roles of the Mise en Pratique for the Definition of the Kelvin

October 16, 2010
Author(s)
Dean C. Ripple, Richard Davis, Bernd Fellmuth, Joachim Fischer, Graham Machin, Terry Quinn, Peter Steur, Osamu Tamura, David R. White
The mise en pratique (“practical realization”) for the definition of the kelvin (MeP-K) was created by the Consultative Committee for Thermometry (CCT) in 2006 to give practitioners of thermometry a guide to the realization of the kelvin, in accord with

Characterization of gold nanoparticles modified with single-stranded DNA using analytical ultracentrifugation and dynamic light scattering

August 3, 2010
Author(s)
James Falabella, Tae Joon Cho, Dean C. Ripple, Vincent A. Hackley, Michael J. Tarlov
We report the characterization of gold nanoparticles modified with thiol-terminated single stranded DNA using analytical ultracentrifugation and dynamic light scattering. Sedimentation coefficients of nominally 10 and 20 nm diameter gold nanoparticles

Thermal Analysis of Refrigeration Systems Used for Vaccine Storage

November 2, 2009
Author(s)
Michal J. Chojnacky, W Wyatt Miller, Dean C. Ripple, Gregory F. Strouse
Each year, billions of dollars of vaccines are stored in refrigerators at the facilities of a variety of medical providers. Many vaccines must be maintained in the range 2 °C to 8 °C to retain product potency. We have tested the performance of two types of

Thermometry Issues in Destruction Kinetics Measurements

June 26, 2009
Author(s)
Dean C. Ripple
Of all the aspects of destruction kinetics measurement, the one taken most for granted is temperature measurement. The ubiquity of digital thermometers and their ease of use have disguised potential accuracy issues in the range of temperature encountered