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Search Publications by: Lili Wang (Fed)

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Displaying 126 - 150 of 157

Effect of Target and Probe Concentrations on Hybridization in DNA Microarrays

June 1, 2004
Author(s)
Lili Wang, Adolfas K. Gaigalas, Y Zong, S Zhang, J Shi, Yunda Wang
Efficient hybridization of complementary strands of DNA is the underlying principle of all microarray-based techniques for gene expression analysis. Recently studies have been published to assess oligonucleotide (55-70 bases) performance on glass-slide

Formalization of the MESF Unit of Fluorescence Intensity

January 1, 2004
Author(s)
A. Schwartz, Adolfas Gaigalas, Lili Wang, G E. Marti, R F. Vogt, R Fernandez-Prini
This report summarizes the work performed during the past two years at the National Institute of Standards and Technology (NIST) in the refinement and formal definition of the MESF unit of fluorescence intensity. In addition to the theory underlying the

Fluorescence Resonance Energy Transfer Between Donor-Acceptor Pair on Two Oligonucleotides Hybridized Adjacently to a DNA Template

January 1, 2003
Author(s)
Lili Wang, Adolfas K. Gaigalas, J L. Blasic, Marcia J. Holden, David T. Gallagher, R Pires
We have used fluorescein as the energy donor and rhodamine as the acceptor to measure the efficiency of fluorescence resonance energy transfer (FRET) in a set of hybridized DNA constructs. The two fluorophores are covalently attached via linkers to two

Frequency-Domain Measurement of the Photodegradation Process of Fluorescein

July 1, 2002
Author(s)
Adolfas K. Gaigalas, Lili Wang, R F. Vogt
The frequency domain technique is applied to measure the photodegrdation rate offluorescein in aqueous solutions. The illuminating light is modulated and the changes in fluorescence from the illuminated region are detected synchronously. A constant flow

Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values

July 1, 2002
Author(s)
Lili Wang, Adolfas K. Gaigalas, F Abassi, G E. Marti, R F. Vogt, A Schwarz
The present work uses fluorescein as the model fluorophore and points out critical steps in the use of MESF (Molecules of Equivalent Soluble Fluorophores) values for quantitative flow cytomeric measurements. It has been found that the emission spectrum

Quantitating Fluorescence Intensity From Fluorophore: The Definition of MESF Assignment

January 1, 2002
Author(s)
A. Schwartz, Lili Wang, E A. Early, Adolfas Gaigalas, Aimin Zhang, G E. Marti, R F. Vogt
The quantitation of fluorescence radiance may at first suggest the need to obtain the number of fluorophore that are responsible for the measured fluorescence radiance. This goal is beset by many difficulties since the fluorescence radiance depends on