U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications by: John Butler (Fed)

Search Title, Abstract, Conference, Citation, Keyword or Author
Displaying 26 - 50 of 203

Evaluation of Forensic DNA Mixture Evidence: Guidelines for Evaluation, Interpretation, and Statistical Calculations using the Combined Probability of Inclusion

August 31, 2016
Author(s)
Frederick R. Bieber, John Buckleton, Bruce Budowle, John Butler, Michael D. Coble
The evaluation and interpretation of forensic DNA mixture evidence faces greater interpretational challenges due to increasingly complex mixture evidence. Such challenges include: casework involving low quantity or degraded evidence leading to allele and

Recent Activities of the National Commission on Forensic Science

July 1, 2016
Author(s)
John Butler
In February 2013, the U.S. Department of Justice (DOJ) and the National Institute of Standards and Technology (NIST) announced a partnership that included formation of the National Commission on Forensic Science (NCFS) and what is now the Organization of

NIJ Funding: Supporting Scientists, Supporting Innovation

December 1, 2015
Author(s)
John Butler
In this invited submission to the NIJ Journal, I reflect on the role that National Institute of Justice (NIJ) funding has played in my career in forensic science. Early experiences at the FBI Laboratory are discussed along with the benefits of

STR Allele Sequence Variation: Current Knowledge and Future Issues

July 6, 2015
Author(s)
Katherine Gettings, Rachel A. Aponte, Peter Vallone, John M. Butler
This article reviews what is currently known about short tandem repeat (STR) allelic sequence variation in and around the twenty-four loci most commonly used throughout the world to perform forensic DNA investigations. These STR loci include D1S1656, TPOX

The Future of Forensic DNA Analysis

June 22, 2015
Author(s)
John M. Butler
The author's thoughts and opinions on where the field of forensic DNA testing is headed for the next decade are provided in the context of where the field has come over the past 30 years. Like the Olympic motto of "faster, higher, stronger", forensic DNA

Advanced Topics in Forensic DNA Typing: Interpretation (textbook)

September 1, 2014
Author(s)
John M. Butler
This book is primarily intended for DNA analysts or those trying to understand what a DNA analyst does in his or her review of forensic DNA data that was obtained by polymerase chain reaction (PCR) amplification and short tandem repeat (STR) typing via

Some Journal Metrics for Forensic Science International: Genetics

October 2, 2013
Author(s)
John M. Butler
Forensic Science International: Genetics, which is the official journal of the International Society for Forensic Genetics, has grown in readership and impact factor since its introduction in 2007. This article reviews some metrics of the 32 issues

The Triad of Scientific Publication: Reading, Writing, and Reviewing

October 2, 2013
Author(s)
John M. Butler
A workshop on scientific publication was presented at the 2013 ISFG conference by the author, who has written four widely-used textbooks and 150 peer-reviewed articles and invited book chapters. This article is a summary of key points made during the

Biology and Genetics of New Autosomal STR Loci Useful for Forensic DNA Analysis

August 19, 2013
Author(s)
John M. Butler, Carolyn R. Steffen
Short tandem repeats (STRs) are regions of tandemly repeated DNA segments found throughout the human genome that vary in length (through insertion, deletion, or mutation) with a core repeated DNA sequence. Forensic laboratories commonly use tetranucleotide

Haplotype Data for 23 Y-chromosome markers in four U.S. population groups

May 1, 2013
Author(s)
Michael D. Coble, Carolyn R. Steffen, John M. Butler
The PowerPlex Y23 kit contains 23 Y-chromosomal loci including all 17 of the markers in the Yfiler Y-STR kit plus six additional markers: DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643. We have typed 1032 unrelated population samples from four self

Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA

March 1, 2013
Author(s)
Ross J. Haynes, Margaret C. Kline, Blaza Toman, Calum Scott, Paul Wallace, John M. Butler, Marcia J. Holden
Cytomegalovirus (CMV) is one of a number of human viruses that are ubiquitous in human populations, generally cause little illness upon infecting healthy individuals, but can cause life threatening disease in immune-compromised individuals. An important

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

January 1, 2013
Author(s)
John Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone, A. Carracedo
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

Forensic DNA Advisory Groups: DAB, SWGDAM, ENFSI, and BSAG

December 28, 2012
Author(s)
John M. Butler
A number of organizations exist around the world that work on a national or international level to aid quality assurance and to promote accurate forensic DNA testing. These organizations are made up primarily of working scientists who coordinate their

Developmental Validation of the PowerPlex(R) ESI 17 Pro System

December 27, 2012
Author(s)
Carolyn R. Steffen, Margaret C. Kline, John Butler, Robert S. McLaren, Jaynish Patel, Margaret Ewing, Douglas R. Storts, Fabrice Noel, Sophie Dognaux
The SE33 locus is one of the most polymorphic markers used in human identification. However, it also possesses multiple microvariants both within the repeat and in the flanking regions. Such flanking region mutations can generate discordant allele calls