Publications

Displaying 26 - 50 of 66

Primary Determination of Particle Number Concentration with Light Obscuration and Dynamic Imaging Particle Counters

January 11, 2018

Author(s)

Dean C. Ripple, Paul C. DeRose

Accurate number concentrations of particles in liquid media are needed to assess the quality of water, pharmaceuticals, and other liquids, yet there are limited reference materials or calibration services available with clear traceability to the

Development of NIST Standard Reference Material 2082, a Pathlength Standard for Measurements in the Ultraviolet Spectrum

July 10, 2017

Author(s)

Brian Lang, Aaron A. Urbas, Paul C. DeRose, Hung-Kung Liu, John C. Travis, Steven J. Choquette, Kenneth D. Cole

New spectrophotometers and cuvettes have been designed to allow the measurement of absorbance values from samples using microliter volume sizes. These measurements are done using short pathlengths to decrease the sample volumes required. The major

Assignment of the Number of Equivalent Reference Fluorophores to Dyed Microspheres

June 2, 2016

Author(s)

Adolfas Gaigalas, Lili Wang, Paul C. DeRose

A procedure will be described to assign to each dyed microsphere a number called the Equivalent number of Reference Fluorophores (ERF). The ERF unit gives the number of reference fluorophores in solution which produce the same fluorescence signal as a

Optical Properties of CdSe/ZnS Nanocrystals

December 31, 2014

Author(s)

Paul C. DeRose, Yu-Zhong Zhang, Adolfas Gaigalas

Measurements are presented of the absorbance, fluorescence emission, fluorescence quantum yield, and fluorescence lifetime of CdSe/ZnS nanocrystals, also known as quantum dots (QDs). The study included three groups of nanocrystals whose surfaces were

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass

August 11, 2014

Author(s)

Michael W. Halter, Elianna Bier, Paul C. DeRose, Gregory A. Cooksey, Steven J. Choquette, Anne L. Plant, John T. Elliott

Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist for

Spectral Fluorescence Measurements

July 7, 2014

Author(s)

Paul C. DeRose, Joanne C. Zwinkels, James Leland

This chapter covers the topic of spectral fluorescence measurements. These fluorescence measurements offer significant advantages in terms of sensitivity and selectivity, finding wide use in a range of applications in analytical and color technologies. The

Quantification of Calcium Dipicolinate in Bacillus Anthracis Spores Using a Beetroot Pigment

September 3, 2013

Author(s)

Paul C. DeRose, Sandra M. Da Silva, Let?a C. Gon?ves, Erick L. Bastos, R?o A. Ando

In this proof-of-concept study, we describe the use of the main red beet pigment betanin for the detection of bacterial spores, including Bacillus anthracis. In the presence of europium(III) ions, betanin is converted to a water-soluble, non-luminescent

Standard Practice for Determining the Relative Spectral Correction Factors for the Emission Signal of Fluorescence Spectrometers

August 15, 2013

Author(s)

Paul C. DeRose

This standard practice is intended to aid in the calibration and qualification of fluorescence spectrometers as part of complying with regulatory and other QA/QC requirements. It specifies the materials and procedures used in three different methods for

Characterization of Standard Reference Material 2944, Bi-Ion-Doped Glass, Spectral Correction Standard for Red Fluorescence

March 13, 2013

Author(s)

Paul C. DeRose, Melody V. Smith, Jeffrey R. Anderson, Gary W. Kramer

Standard Reference Material (SRM) 2944 is a cuvette-shaped, Bi-ion-doped glass, recommended for use for relative spectral correction of emission and day-to-day performance verification of steady-state fluorescence spectrometers. Properties of this standard

State-of-the-Art Comparability of Corrected Emission Spectra-Part II: Peer-toPeer Assessment of Calibration Performance Using Spectral Fluorescence Standards

February 28, 2012

Author(s)

Paul C. DeRose, Joanne C. Zwinkels, Bernd Ebert

The tremendous growth of fluorescence applications in the life and material sciences has proceeded largely without sufficient concern for the reliability and uncertainties related to the characterization and performance validation of fluorescence

Charaterization of Standard Reference Material 2943, Cu-Ion-Doped Glass, Spectral Correction Standard for Blue Fluorescence

December 1, 2011

Author(s)

Paul C. DeRose, Melody V. Smith, Klaus Mielenz, Jeffrey R. Anderson, Gary W. Kramer

Standard Reference Material (SRM) 2943 is a cuvette-shaped, Cu0ion-doped glass, recommended for use for relative spectral correction of emission and day-to-day performance verification of steady-state florescence spectrometers. Properties of this standard

Characterization of Standard Reference Material 2942, Ce-Ion-Doped Glass, Spectral Correction Standard for UV Fluorescence

March 8, 2011

Author(s)

Paul C. DeRose, Melody V. Smith, Klaus Mielenz, Jeffrey R. Anderson, Gary W. Kramer

Standard Reference Material (SRM) 2942 is a cuvette-shaped, Ce-ion-doped glass, recommended for use for relative spectral correction of emission and day-to-day performance verification of steady-state fluorescence spectrometers. Properties of this standard

Recommendations for Fluorescence Instrument Qualification: The New ASTM Standard Guide

March 1, 2010

Author(s)

Paul C. DeRose, Ute Resch-Genger

Aimed at improving quality assurance and quantitation for modern fluorescence techniques, ASTM is about to release a Standard Guide for Fluorescence, reviewed here. The guide s main focus is on steady state fluorometry, for which available standards and

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