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Search Publications by: Alessandro Tona (Fed)

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Displaying 26 - 50 of 69

High Resolution Surface Plasmon Resonance Imaging for Single Cells

November 30, 2014
Author(s)
Alexander Peterson, Michael Halter, Alessandro Tona, Anne Plant
Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However

Identification and Quantification of DNA Repair Protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) in Human Cells by Liquid Chromatography/Isotope-Dilution Tandem Mass Spectrometry

July 29, 2013
Author(s)
Guldal Kirkali, Pawel Jaruga, Prasad T. Reddy, Alessandro Tona, Li Mengxia, David M. Wison III, M Miral Dizdar, Bryant C. Nelson
Unless repaired, DNA damage can drive mutagenesis or cell death. DNA repair proteins may therefore be used as biomarkers in disease detection or therapeutic response estimation. Thus, the accurate measurement of DNA repair protein expression is of

Simple device for rare cell capture from whole blood

September 26, 2012
Author(s)
Jason G. Kralj, Samuel P. Forry, Matt S. Munson, Thomas P. Forbes, Chandamany Arya, Lynn Sorbara, Alessandro Tona, Sudhir Srivastava
We have developed a system to isolate rare cells from whole blood using commercially available components and simple microfluidics that can provide biologists with the capabilities of the monolithic devices. We characterized the capture of MCF-7 cells

Quantitative Methods to Characterize Cell Lines: Comparison of Cells from Marine and Terrestrial Mammals.

July 28, 2012
Author(s)
Tighe Spurlin, John T. Elliott, Michael W. Halter, Kiran Bhadriraju, Alessandro Tona, Anne L. Plant, Annalaura Mancia, Bobby L. Middlebrooks, Gregory W. Warr
Descriptive terms are often used to characterize cells in culture, but the use of nonquantitative and poorly defined terms can lead to ambiguities when comparing data from different laboratories. Although recently there has been a good deal of interest in

A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP

August 1, 2009
Author(s)
Michael W. Halter, Jamie L. Almeida, Alessandro Tona, Kenneth D. Cole, Anne L. Plant, John T. Elliott
Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that provides a

Cell volume distributions reveal cell growth rates and division times

March 7, 2009
Author(s)
Michael W. Halter, John T. Elliott, Joseph B. Hubbard, Alessandro Tona, Anne L. Plant
A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable rates

Surface plasmon resonance imaging of cells and surface-associated fibronectin

February 26, 2009
Author(s)
Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Kiran Bhadriraju, Anne L. Plant
Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for