Publications

Displaying 26 - 50 of 86

Histone post-translational modifications in frontal cortex from human donors with Alzheimers disease

October 1, 2015

Author(s)

Kyle W. Anderson, Illarion V. Turko

Histone H3 Ser57 and Thr58 Phosphorylation in 5XFAD Mouse Brain

June 20, 2015

Author(s)

Kyle W. Anderson, Illarion V. Turko, Natalia Mast, Irina A. Pikuleva

Alzheimers disease (AD) is the most common neurodegenerative disorder, yet it is poorly understood. Currently, there is no treatment to delay onset or slow progression of AD. Epigenetics of AD is a growing field that has gained interest due to the

Quantification of Histone Deacetylase Isoforms in Human Frontal Cortex, Human Retina, and Mouse Brain

May 11, 2015

Author(s)

Kyle W. Anderson, Illarion V. Turko, Meiyao M. Wang, Junjun J. Chen, Irina A. Pikuleva, Natalia Mast

Histone deacetylases (HDACs) are primarily responsible for the deacetylation of histones, thereby regulating gene expression, as well as modifying many non-histone proteins. HDAC inhibition has promise as a therapy for Alzheimers disease (AD) and other

Quantitative performance of internal standard platforms for absolute protein quantification using MRM-MS

March 26, 2015

Author(s)

Kerry M. Bauer, Illarion V. Turko, Karen W. Phinney

Isotope dilution mass spectrometry involves the addition of known quantities of isotope-labeled standards, that mimic native molecules, to biological samples. We evaluated three conventional internal standard platforms (synthetic peptides, QconCAT

Natural Flanking Sequences for Peptides Included in Quantification Concatamer Internal Standard

January 20, 2015

Author(s)

Sao F. Cheung, Kyle Anderson, Meiyao Wang, Illarion Turko

Quantification by targeted proteomics has largely depended on mass spectrometry and isotope-labeled internal standards. In addition to traditionally used recombinant proteins or synthetic peptides, concatenated peptides (QconCATs) were introduced as a

Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

December 11, 2014

Author(s)

Meiyao M. Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Jeffrey M. Davis, Kenneth D. Cole, Illarion Turko, Lili Wang, Fatima Abbasi

For quantitative flow cytometry, a biological cell reference material with a known biomarker expression level is needed to transform a linear arbitrary fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per cell (ABC)

Trends in QconCATs for targeted proteomics

May 1, 2014

Author(s)

Junjun J. Chen, Illarion Turko

Targeted proteomics has received much attention because of the highly-sensitive, quantitative detection of proteins and post-translational modifications (PTMs). Quantification by targeted proteomics relies on mass spectrometry and isotope-labeled internal

Structural Basis for Inactivation of Giardia lamblia Carbamate Kinase by Disulfiram

April 11, 2014

Author(s)

Andrey Galkin, Liudmila Kulakova, Kap Lim, Catherine Chen, Wei Zhang, Illarion Turko, Osnat Herzberg

Carbamate kinase from Giardia lamblia is an essential enzyme for the survival of the organism. The enzyme catalyzes the final step in the arginine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate. We previously reported that

Determining carbapenemase activity with 18O labeling and targeted mass spectrometry

October 16, 2013

Author(s)

Meiyao Wang, Yang Shen, Illarion Turko, Daniel Nelson, Shuwei Li

Carbapenems are broad spectrum antibiotics considered as last resort drugs to treat bacterial infections. Carbapenem-hydrolyzing β-lactamases (also called carbapenemases), however, can confer bacterial resistance and represent a serious health threat. Here

Pretreatment with Pyridoxamine Mitigates Isolevuglandinassociated Retinal Effects in Mice Exposed to Bright Light

October 11, 2013

Author(s)

Casey Charvet, Aicha Saadane, Meiyao M. Wang, Robert Salomon, Henri Brunengraber, Christine Curcio, Illarion Turko, Irina Pikuleva

The benefits of antioxidant therapy for treating age-related macular degeneration, a devastating retinal disease, are limited. Perhaps species other than reactive oxygen intermediates should be considered as therapeutic targets. These could be lipid

Quantification of transferrin in human serum using both QconCAT and synthetic internal standards

September 27, 2013

Author(s)

Tyler A. Zimmerman, Meiyao M. Wang, Mark S. Lowenthal, Illarion V. Turko, Karen W. Phinney

Transferrin, an iron transport protein, is a clinically-important biomarker in diseases such as iron-deficiency anemia. Current diagnostic methods for transferrin levels lack quantitative accuracy, suggesting the need for alternative approaches like LC-MS

Mass spectrometry quantification of PICALM and AP180 in human frontal cortex and neural retina

August 13, 2013

Author(s)

Illarion V. Turko, Junjun J. Chen, Irina Pikuleva

Recent genome-wide association studies have suggested that endocytic factors, such as phosphatidylinositol-binding clathrin assembly protein (PICALM), may be implicated in the development of Alzheimer disease (AD). The cellular functions of PICALM are in

Mass Spectrometry Assessment of Ubiquitin Carboxyl-Terminal Hydrolase L1 Partitioning between Soluble and Particulate Brain Homogenate Fractions

May 20, 2013

Author(s)

Junjun J. Chen, Richard Huang, Illarion Turko

Partitioning of specific proteins between soluble and insoluble forms because of aggregation, membrane attachment, and (or) association with senile plaques and neurofibrillary tangles is a major feature of several neurodegenerative disorders, including

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