Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications by: Jason Kralj (Fed)

Displaying 26 - 28 of 28

T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements

April 7, 2009

Author(s)

Jason G. Kralj, Audrey Player, Hope Sedrick, Matt S. Munson, David Petersen, Samuel P. Forry, Paul Meltzer, Ernest Kawasaki, Laurie E. Locascio

We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microarray

Multiplexed "Detectorless" Electrophoresis

December 14, 2008

Author(s)

David Ross, Jason Kralj

A new microfluidic electrophoresis device and technique is described that is designed specifically for multiplexed, high throughput separations. The device consists of an array of short (3 mm) capillaries connecting individual sample reservoirs to a common