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Search Publications by: Steven Lund (Fed)

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Displaying 1 - 25 of 51

Inconclusive Decisions and Error Rates in Forensic Science

May 4, 2024
Author(s)
Henry Swofford, Steven Lund, Hariharan K. Iyer, John Butler, Johannes A. Soons, Robert M. Thompson, Vincent Desiderio, JP Jones, Robert Ramotowski
In recent years, there has been discussion and controversy relating to the treatment of inconclusive decisions in forensic feature comparison disciplines when considering the reliability of examination methods and results. In this article, we offer a brief

Inconclusive Decisions and Error Rates in Forensic Science

May 4, 2024
Author(s)
Henry Swofford, Steven Lund, Hariharan Iyer, John Butler, Johannes Soons, Robert Thompson, Vincent Desiderio, JP Jones, Robert Ramotowski
Conference Presentation based on technical paper in ERB (Control Number: N2024-0457).

Effects of DNA Template Preparation on Variability in Cell-Free Protein Production

August 13, 2022
Author(s)
Eugenia Romantseva, Nina Alperovich, David Ross, Steven Lund, Elizabeth Strychalski
DNA templates for protein expression remain an unexplored source of variability in the performance of cell-free expression (CFE) systems for protein production. To characterize this variability, we investigated the effects of two common DNA extraction

Quantitative Evaluation of Footwear Evidence: Initial Workflow for an End-to-End System

November 23, 2021
Author(s)
Gautham Venkatasubramanian, Vighnesh Hegde, Steven Lund, Hariharan K. Iyer, Martin Herman
In the U.S., footwear examiners make decisions about the sources of crime scene shoe impressions using subjective criteria. This has raised questions about the accuracy, repeatability, reproducibility, and scientific validity of footwear examinations

Accurate and Robust Trypan Blue-Based Cell Viability Measurement Using Neural Networks

July 19, 2021
Author(s)
Adele Peskin, Steven Lund, Chenyi Ling, Laura Pierce, Sumona Sarkar, Firdavs Kurbanov, Michael Halter, Joe Chalfoun, John T. Elliott
Trypan blue dye exclusion-based cell viability measurements are highly dependent upon image quality and consistency. In order to make measurements repeatable, one must be able to reliably capture images at a consistent focal plane, and with signal-to-noise

Examining Recent HR Data for Gender Bias Among Federal Employees at NIST

March 26, 2021
Author(s)
Jasmine Evans, Amanda Koepke, Steven Lund, Mary Frances Theofanos
This report is part of a multi-phase mixed methods project that is designed to provide an in-depth look at the population of National Institute of Standards and Technology (NIST) federal employees to identify factors, attitudes, and processes that might

The Roles of Standard Cigarettes in Assuring the Ignition Resistance of Soft Furnishings

November 18, 2020
Author(s)
Richard G. Gann, Ickchan Kim, Steven P. Lund, William F. Guthrie, Rick D. Davis
Regulations for cigarette ignition resistance (CIR) of soft furnishings (beds and upholstered furniture) and less fire-prone cigarettes have contributed substantially to the decrease in losses from cigarette-initiated fires over time. Two Standard

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines

June 20, 2019
Author(s)
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah L. Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin Hall, Mark Ladd, Megan Shaffer, Heath Berg, Jinliang Li, Georges Wigger, Steven P. Lund, Carolyn R. Steffen, Barbara Fransway, Bob Geraghty, Manuela Natoli, Beth Bauer, Susanne M. Gollin, Dale Lewis, Yvonne A. Reid
The Consortium for Mouse Cell Line Authentication was formed to validate Short Tandem Repeat (STR) markers for intraspecies identification of mouse cell lines. The STR profiling method is a multiplex polymerase chain reaction (PCR) assay comprised of

Development and Interlaboratory Evaluation of a NIST Reference Material RM 8366 for EGFR and MET Gene Copy Number Measurements

May 21, 2019
Author(s)
Hua-Jun He, Biswajit Das, Megan H. Cleveland, Chen Li, Corinne Camalier, Liang-Chu Liu, Kara L. Norman, Andrew Fellowes, Christopher McEvoy, Steven P. Lund, Jamie L. Almeida, Carolyn R. Steffen, Chris Karlovich, P. M. Williams, Kenneth D. Cole
The National Institute Standard and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR (epidermal growth factor receptor) and MET (proto-oncogene, receptor tyrosine kinase), important targets

Counting Caenorhabditis elegans: Protocol Optimization and Applications for Population Growth and Toxicity Studies in Liquid Medium

January 17, 2018
Author(s)
Steven Lund, Sanem Hosbas Coskun, Monique Johnson, Christopher Sims, Elijah Petersen, John T. Elliott, Bryant C. Nelson, Leona D. Scanlan, Shannon K. Hanna, Karina Brignoni, Patricia Lapasett
The nematode Caenorhabditis elegans is used extensively in molecular, toxicological and genetics research. However, standardized methods for counting nematodes in liquid culture do not exist despite the wide use of nematodes and need for accurate

Likelihood Ratio as Weight of Forensic Evidence: A Closer Look

October 12, 2017
Author(s)
Hariharan K. Iyer, Steven P. Lund
The forensic science community has increasingly sought quantitative methods for conveying the weight of evidence. Experts from many forensic laboratories summarize their fndings in terms of a likelihood ratio. Several proponents of this approach have

Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next- Generation Sequencing Methods

September 1, 2016
Author(s)
Chih-Jian Lih, Robin D. Harrington, Kneshay Harper, David J. Sims, Paul McGregor, Corinne Camalier, Han Si, Biswajit Das, P. M. Williams, Hua-Jun He, Jamie L. Almeida, Steven Lund, Steven J. Choquette, Kenneth D. Cole
The National Institute of Standards and Technology (NIST) Standard Reference Materials 2373 is a set of genomic DNA samples prepared from five breast cancer cell lines with certified values for the ratio of the HER2 gene copy number to the copy numbers of