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Search Publications by: Paul Patrone (Fed)

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Displaying 1 - 25 of 79

Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection

October 28, 2023
Author(s)
Lili Wang, Paul Patrone, Anthony Kearsley, Jerilyn Izac, Adolfas Gaigalas, John Prostko, Hang Xie, Linhua Tian, Elzafir Elsheikh, Edward Kwee, Troy Kemp, Simon Jochum, Natalie Thornburg, Clifford McDonald, Adi Gundlapalli, Sheng Lin-Gibson
COVID-19 has highlighted the need for more accurate and reproducible measurements of humoral immunity, including antibody levels and neutralization potential, which are critical for diagnostics, seroprevalence estimates, and development of vaccines and

Optofluidic Flow Meter for sub-nanoliter per minute flow measurements

January 31, 2022
Author(s)
Jalal Sadeghi, Paul Patrone, Anthony J. Kearsley, Gregory A. Cooksey
Performance improvements in microfluidic systems depend on accurate measurement and control of fluids on the micro- and nanoscale, and new applications are continuously moving the needle to lower volumetric flow rates. This work focuses on improving an

CD19 Expression Analysis Using Two Flow cytometric Quantitative Schemes: An approach for Identifying Robust B-cell Reference Control Materials

March 19, 2021
Author(s)
Hao-Wei Wang, Paul Patrone, Anthony Kearsley, Jessica Watson, Liang Lim, Jothir Pichaandi, Olga Ornatsky, Daniel Majonis, Rukmini Bhardwaj, Howard Mostowski, Steven Bauer, Heba Degheidy, Lili Wang
Cell-based therapies have emerged as a novel approach to treat cancer and other conditions. Quantitative flow cytometry plays an increasingly important role in predicting therapy outcome in a wide range of hematologic malignancies. Several studies

Measurement of serological and neutralizing antibodies specific to SARS-CoV-2 virus in humans with COVID-19 disease

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
The global response to COVID-19 has led to rapid advances in diagnostic, surveillance, and vaccine development. Critical measurements underpinning many of these efforts include serological assays to assess seroprevalence of populations and the complex

Failure mechanisms in DNA self-assembly: Barriers to single fold yield

February 23, 2021
Author(s)
Jacob Majikes, Paul Patrone, Anthony J. Kearsley, Michael P. Zwolak, James Liddle
Understanding the folding process of DNA origami is a critical steppingstone to the broader implementation of nucleic acid nanofabrication technology but is notably non-trivial. Origami are formed by several hundred cooperative hybridization events – folds

Matching and comparing objects in a serial cytometer

October 4, 2020
Author(s)
Nikita Podobedov, Matthew DiSalvo, Jason A. Hsu, Paul Patrone, Gregory A. Cooksey
Flow cytometers are indispensable for clinical studies, yet are hindered by inherent uncertainties. We have developed an optofluidic device capable of multiple measurements along a microfluidic channel, whereby many of the uncertainty components can be

Serial microfluidic cytometry with inertial and hydrodynamic flow focusing

October 4, 2020
Author(s)
Matthew DiSalvo, Paul Patrone, Gregory A. Cooksey
Microfluidics are increasingly used to develop flow cytometers with novel functionalities. Although various approaches exist to control particle positioning within microfluidics, the magnitude and mechanisms of measurement uncertainties that arise from