Selective Streptavidin Bioconjugation on Si, SiC, and GaN Nanowires for Biosensor Applications.

Published

October 19, 2012

Author(s)

Elissa H. Williams, John A. Schreifels, Mulpuri V. Rao, Albert Davydov, Vladimir Oleshko, Nancy Lin, Kristen L. Steffens, Sergiy Krylyuk, Kristine A. Bertness, Amy Manocchi, Yaroslav Koshka

Abstract

A functionalization method for the specific and selective immobilization of the streptavidin (SA) protein on semiconductor nanowires (NWs) was developed. Silicon (Si), silicon carbide (SiC), and gallium nitride (GaN) NWs were functionalized with 3-aminopropyltriethoxysilane (APTES) and subsequently biotinylated for the conjugation of SA. Field-emission scanning electron microscopy (FESEM), X-ray photoelectron spectroscopy (XPS), high-resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM), and fluorescence microscopy were used to validate each surface functionalization step. It was shown that there was significant non-specific, electrostatic binding of the SA and the bovine serum albumin (BSA) protein to APTES coated Si, SiC, and GaN NWs. Inhibition of BSA and enhancement of SA binding was achieved only on the biotinylated NWs. The biofunctionalized semiconductor NWs have the potential to be employed as multiplex biosensing platforms for specific and selective detection of protein molecules.

Citation

Journal of Materials Research

Volume

8467

Pub Type

Journals

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DOI Link

Keywords

biosensor, nanowires, surface chemistry

Citation

Williams, E. , Schreifels, J. , Rao, M. , Davydov, A. , Oleshko, V. , Lin, N. , Steffens, K. , Krylyuk, S. , Bertness, K. , Manocchi, A. and Koshka, Y. (2012), Selective Streptavidin Bioconjugation on Si, SiC, and GaN Nanowires for Biosensor Applications., Journal of Materials Research, [online], https://doi.org/10.1117/12.970460 (Accessed December 26, 2024)