Balss, K.
, Ross, D.
and Tarlov, M.
(2004),
Temperature Gradient Focusing of Matched and Partially Mismatched DNA/PNA Hybridizations, International Conference on Miniaturized Chemical and BioChemical Analysis Systems
(Accessed December 26, 2024)
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Temperature Gradient Focusing of Matched and Partially Mismatched DNA/PNA Hybridizations
Published
Author(s)
, ,
Abstract
The hybridization reactions of DNA with peptide nucleic acids (PNA) were monitored in real time with Temperature Gradient Focusing (TGF). Both ex situ and in situ reactions of a perfect complement and mismatched ssDNA targets with PNA were monitored by fluorescence microscopy. TGF was used to first spatially focus a plug of ssDNA within a microchannel and then to hold the plug stationary for hybridization with a peptide nucleic acid probe transported by bulk flow of the buffer. The mismatched ssDNA target did not react with the PNA. TGF of PNA/DNA demonstrated a 500-fold increase in concentration during a 20 minute experiment.Citation
International Conference on Miniaturized Chemical and BioChemical Analysis Systems
Volume
126
Issue
41
Pub Type
Journals
Keywords
concentration method, DNA hybridizations, peptide nucleic acid (PNA), separations
Citation
Issues
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Created October 20, 2004, Updated February 17, 2017