Validation of a Rapid GC-MS Method for Forensic Seized Drug Screening Applications

Published

September 14, 2024

Author(s)

Briana Capistran, Edward Sisco

Abstract

With the lack of standardized validation protocols across the forensic chemistry community, validation of instrumentation can be a challenging and time-consuming task. However, this process is crucial to understanding the associated capabilities and limitations, especially for nascent technologies. Rapid GC-MS is one such emerging analytical technique being increasingly implemented in forensic laboratories due to its fast and informative screening capabilities. However, a full validation for forensic samples has yet to be published since its debut. This work presents the results of a comprehensive validation of a rapid GC-MS system for seized drug screening through the assessment of nine components: selectivity, matrix effects, precision, accuracy, range, carryover/contamination, robustness, ruggedness, and stability. Single- and/or multi-compound test solutions of commonly encountered seized drug compounds were used to assess method and system performance. Results met the designated acceptance criteria for a majority of components. For example, retention time and mass spectral search score % RSDs were ≤ 10 % for precision and robustness studies. Limitations were identified for components that did not meet the acceptance criteria (e.g., inability to differentiate some isomers). The study design is part of a larger validation package developed for rapid GC-MS that includes a validation plan and automated workbook. The template, available for adoption by laboratories, ultimately aims to reduce the barrier of implementation for rapid GC-MS technology.

Citation

Forensic Chemistry

Volume

41

Pub Type

Journals

Download Paper

https://doi.org/10.1016/j.forc.2024.100609

Local Download

Keywords

GC-MS, Validation, Seized drugs, Screening

Citation

Capistran, B. and Sisco, E. (2024), Validation of a Rapid GC-MS Method for Forensic Seized Drug Screening Applications, Forensic Chemistry, [online], https://doi.org/10.1016/j.forc.2024.100609, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=958023 (Accessed November 21, 2024)