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Cycle Threshold

Cycle number (in quantitative PCR) at which the fluorescence generated within a reaction exceeds a defined threshold; this value is converted to a DNA concentration for each sample tested using a standard curve developed from DNA samples of known concentrations.

Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 116 Standard for Training in Forensic DNA Quantification Methods. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2020. Source link