Polymerase Chain Reaction (Pcr)
An enzymatic process by which a specific region of DNA is replicated during repetitive cycles that consist of the following: denaturation of the template; annealing of primers to complementary sequences at an empirically determined temperature; and extension of the bound primers by a DNA polymerase. The goal of the PCR process is to generate many copies (termed products or amplicons) of a specific region of DNA for further analysis.
Committee
Human Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 116 Standard for Training in Forensic DNA Quantification Methods. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2020.
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