Polymerase Chain Reaction (Pcr)
An enzymatic process by which a specific region of DNA is replicated during repetitive cycles that consist of the following: denaturation of the template; annealing of primers to complementary sequences at an empirically determined temperature; and extension of the bound primers by a DNA polymerase. The goal of the PCR process is to generate many copies (termed products or amplicons) of a specific region of DNA for further analysis.
Committee
Human Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 130 Standard for Training in Forensic DNA Amplification Methods for Subsequent Capillary Electrophoresis Sequencing. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2021.
Source link