Primer
A short polynucleotide chain, usually 1830 bases long, which targets a specific region of the template DNA and allows a DNA polymerase to initiate synthesis of a complementary strand; two primers are required for amplification of a particular section of double-stranded DNA. The primers are complementary to opposite strands and are designed to bracket the region of interest for amplification. Polymerization is initiated at the 3' end of the primer and extends in a 5 to 3 manner.
Committee
Wildlife Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 047 Wildlife Forensics Validation Standard - Validating New Primers for Sequencing. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2019.
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