Spectral Calibration
An examination of the contribution of overlap in the emission spectrum of fluorescent dyes used for a specific DNA test on a capillary electrophoresis instrument; permits the color deconvolution necessary for multi-color STR typing or sequencing to be performed; a poor spectral calibration may cause artifact peaks or inaccurate peak height determinations.
Committee
Human Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 115 Standard for Training in Forensic Short Tandem Repeat Typing Methods using Amplification, DNA Separation, and Allele Detection. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2020.
Source link