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Bioscience

Cell measurements

Projects/Programs

Keyword

Organizational Unit

Evaluating Measurement Quality of Microfluidic Cell-based Assays

Completed

Impact and Customers Our work establishes metrics for measurement quality and reproducibility for cell-based assays within microfluidic devices. Many novel microfluidic devices are being created that permit new measurements or enable new biological functions to be tested; however, characterizations...

Evaluating the Quality of Cell Counting Methods: Experimental Design and Statistical Analysis

Ongoing

Related Standards/Tools ISO 20391-2:2019 Biotechnology — Cell counting — Part 2: Experimental design and statistical analysis to quantify counting method performance COMET (Counting Method Evaluation Tool) A Shiny Application that executes the statistical analysis and reporting outlined in ISO 20391...

Flow Cytometry Lab

Ongoing

Current Capabilities ERF value assignment measurement service for traceable ERF value assignment to microparticles, Aseptic sorting of cell lines, and primary cells Quantitative measurements of cell surface and intracellular biomarkers by estimating antibody bound per cell Accurate enumeration of...

Fluorescence Microscopy Benchmarking: Overview

Completed

A challenge in using microscopy is how to compare and validate data from different instruments, and how to determine that the microscope performance today is the same as yesterday. NIST is developing a practical, automated approach to implementing performance benchmarking methods that will...

Fundamental Interaction Mechanisms of Engineered Nanomaterials with DNA

Completed

Intended impact Nanotechnology research has resulted in the rapid creation of engineered nanomaterials with many foreseeable applications in medical imaging/diagnosis and in drug delivery. However, there is a notable scarcity of both acute and chronic human toxicity data for these new materials...

Improved Benchtop Connectivity to Complex Microfluidic Devices

Completed

Intended impact Microfluidic devices with many fluidic and pneumatic inlets are difficult to connect to benchtop reservoirs and controllers. Many hours can be spent sorting, untangling, and connecting tubing to a single device. If one then finds that the device is not fully functional, the process...

Integrated Lentiviral Vector Copy Number Measurements: Invitation to Participate in an Interlaboratory Testing Program Evaluating Genomic DNA Test Materials

Ongoing

NIST has produced genomic DNA test materials from transduced Jurkat cell lines with integrated lentiviral vector copy number (VCN) for evaluation as a candidate reference material. Transducing Jurkat (Clone E6-1) cell lines with a lentivirus expressing a green fluorescent protein were produced by...

Label-free imaging of cells and their extracellular matrix by SPR imaging

Ongoing

Cellular remodeling of their neighboring environment, extracellular matrix (ECM), is an important biological process from development biology, to wound healing, to diseases and cancers – this is a challenging process to measure and quantify. Surface plasmon resonance imaging (SPRI) has been...

Large-Scale Genotype-Phenotype Landscape Measurements for Precision Engineering of Living Measurement Systems

Ongoing

Synthetic biology promises an array of world-changing innovations including living therapeutics that fight infections and cancer or even regrow whole organs in situ; cellular factories that weave materials with nanoscale features; and synthetic ecosystems that produce fertilizer and pesticides on...

Live Cell Imaging of Induced Pluripotent Stem Cell Populations

Ongoing

As part of our program in Cell Biology , our live cell image program supports the advancement of induced pluripotent stem cell (iPSC) technology in three ways: 1) Identification of process control measurements: A critical component to the translation of iPSCs into therapeutic applications is to...

MML researchers develop reference materials and methods for quantitative phase microscopy with off-the-shelf products

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

Ongoing

Light microscopy is unparalleled as a quantitative technique for studying cell biology, but assuring that the results of measurements and analyses are accurate, repeatable and sharable is challenging. Here we provide information on reference materials, protocols and approaches for evaluating the...

Measurement of Low Frequency Mutations in Circulating Tumor DNA (ctDNA) through Interlaboratory Testing

Completed

A very exciting development in the diagnosis and therapeutic monitoring of cancer is the discovery that blood and urine contain nucleic acids derived from cancer cells that can be used to as biomarkers. DNA, RNA, and microRNA derived from tumor cells can be measured with high sensitivity techniques...

Measurements for minimal cells

Ongoing

Minimal cells are cells whose genome has been stripped of all but those genes essential for replication, in a process called "genome minimization." NIST and collaborators demonstrated genome minimization coupled to single cell imaging as a powerful approach to investigate the minimal genetic...

Measurements and Standards for AI-Ready Biological Data: Unlocking Protein Function

Ongoing

To address this need, the NIST Cellular Engineering Group is collaborating with the Align Foundation and several other laboratories to develop a framework of measurements and standards for AI-ready data. This framework is based on four principles designed to maximize the impact of data collection...

Measurements and Theory for Predicting Stem Cell Stability

Completed

Cell-based therapies require efficient manufacturing processes, as well as assurance of the safety and the stability of cellular products. However, our ability to predict the response of cells to environmental conditions is far from perfect. To move beyond empirical approaches, we are using...

Measuring Cell Viability in Scaffolds

Ongoing

Project Goals Primary Goal: Advance measurements of cell viability in manufactured tissues Establish working group of industry stakeholders to guide work Develop model scaffold-cell-assay system for measuring cell viability in scaffolds Validate measurement system by orthogonal test methods Assess...

Methods to determine the evolutionary stability of engineered biological function

Ongoing

Advances in engineering biology have greatly expanded our ability to add new and useful functions to living cells. Engineering stable functions has remained a significant challenge, with many different approaches developed to increase the stability, or decrease the variability, of a function over...

Methods and Measurements of Nanoparticle Distribution in the Natural Environment

Completed

Intended Impact Understanding the potential environmental fate of NPs has direct implications to the US economy, as potential environmental and human health impacts of NPs is one of the main factors slowing the commercialization of this technology. Reducing the uncertainty regarding the...

Gears illustrating NIST's cell-free systems initiatives

Metrology For Cell-Free Expression Systems

Ongoing

Cell-free expression systems are poised to advance the US bioeconomy and play a key role in ensuring US manufacturing resilience. NIST has several, on-going projects in its growing portfolio to promote reproducibility, new measurement methods, and best practices and standards in cell-free expression...

Microbial Flow Cytometry

Ongoing

Microbial flow cytometry capabilities support numerous ongoing projects, including: Microbial Cell Enumeration and Characterization: Establishing standards, measurement methods, and Reference Materials to enable stakeholders to assess accuracy, bias, and comparability across microbial enumeration...

Figure showing four components of metabolomics with samples and processing

Microbial Metabolomics

Ongoing

Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are widely used and complementary analytical platforms for the characterization, identification, and quantification of a metabolome. MS techniques, typically coupled to chromatography, provide a chemical fingerprint of complex...

Mouse Allelic Ladder

Ongoing

What is an allelic ladder? An allelic ladder is a mixture of highly characterized alleles (known size and sequence) that are generated using the same primers as the test samples and are used during capillary electrophoresis for accurate allele call determination. Why is an allelic ladder important...

Multiparametric Measurement and Analysis of Cell Behavior on Extracellular Matrices

Completed

The rational design of biomaterials which can elicit desired cellular responses is an important requirement for tissue engineering. Understanding the role of different cues that extracellular matrices provide cells is an important component of achieving this objective. Type 1 collagen is a...

Next Generation Metrics for Cell-Based Therapies and Regenerative Medicines

Ongoing

The science of human health and disease often includes studies of cells, the smallest unit of biological complexity. Microscopic imaging is an important tool in these studies and can provide morphological and molecular information. Molecular microscopic data is most often collected in a static mode...

NIST/EDRN Cancer Biomarker Collaboration

Completed

Our lab is one of the EDRN Biomarker Reference Laboratories working with the EDRN discovery labs to improve the quality of the measurements done on new cancer biomarkers for early detection of cancer and for cancer risk. TECHNICAL APPROACH Evaluate current assays used for detection of cancer...

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